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Multiple forms of phosphoenolpyruvate carboxylase in mesophyll, epidermal and guard cells of Vicia faba

Multiple forms of phosphoenolpyruvate carboxylase in mesophyll, epidermal and guard cells of Vicia faba

Physiologia Plantarum 86(2): 315-321

The distribution of phosphoenolpyruvate carboxylase (PEPCase, EC in different leaf-cell types and tissues of Vicia faba L. cv. 3-fach Wei-beta-e was studied. The highest specific PEPCase activity was found in guard cell protoplasts (16.3 mu-mol mg-1 protein h-1) whereas for epidermal and mesophyll protoplasts remarkably lower specific activities were found (1.6 and 1.0 mu-mol mg-1 protein h-1, respectively). On chlorophyll and protoplast basis, a similar distribution of enzyme activity was observed. Compared with epidermal extracts, the specific PEPCase activity of mesophyll tissue was 17-fold lower. Immunological studies with polyclonal antibodies to PEPCase indicated 3 immunoreactive proteins in epidermal tissue and guard cell protoplasts with molecular masses of 107,000, 110,000, and 112,000. Only the M-r 107,000 protein was found in extracts of mesophyll and epidermis protoplasts. Western immunoblots after native electrophoresis of epidermal and mesophyll proteins showed a significant difference in PEPCase mobility. It is assumed, that the immunostained proteins of M-r 110,000 and 112,000 represent isoforms or subunits of the PEPCase and that they are involved in stomatal movements.

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Accession: 002438316

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DOI: 10.1034/j.1399-3054.1992.860219.x

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