Pregnancies after transfer of sheep embryos produced from oocytes matured and fertilized in vitro
Slavík, T.; Fulka, J.
Folia Biologica 37(2): 94-100
1991
ISSN/ISBN: 0015-5500
PMID: 1874343
Accession: 002464159
Oocytes isolated from follicles 2-5 mm in diameter were matured in culture for 22-24 h, and inseminated with spermatozoa pretreated with heparin. Of fertile oocytes, 77-100% were monospermic, and 60-68.3% cleaved to the 2-4 cell stage after 24-50 h of culture.
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