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Production of monoclonal anti-idiotype antibodies which mimic an M-like protein of Streptococcus equi

Production of monoclonal anti-idiotype antibodies which mimic an M-like protein of Streptococcus equi

Microbiology and Immunology 37(9): 737-742

Rat monoclonal anti-idiotype antibodies (mAb2) were raised against two mouse monoclonal antibodies (mAb1), 1D10 and 2A6, with specificity for the M-like protein of Streptococcus equi. The capacity of the mAb2 to inhibit the binding between the corresponding mouse mAb1 against which the mAb2 were raised and the M-like protein was investigated in an inhibition EIA. One of the ten mAb2 examined, namely 5D1 (anti-mAb1 1D10), was able to inhibit this binding. The mAb2 5D1 bound to the mAb1 1D10 in such a way as to completely inhibit the subsequent binding of the M-like protein antigen to the paratope of the mAb1 1D10. The mAb2 5D1 is likely to represent a true image of the M-like protein antigen and may thus be described as an Ab2-beta anti-idiotype antibody.

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Accession: 002468001

Download citation: RISBibTeXText

PMID: 8271975

DOI: 10.1111/j.1348-0421.1993.tb01699.x

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