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Production of monoclonal antibodies to Clavibacter michiganense subsp. sepedonicum

Production of monoclonal antibodies to Clavibacter michiganense subsp. sepedonicum

Acta Phytopathologica Sinica 23(1): 11-16

Cell wall protein extract from str. HECL of the potato ring rot pathogen, C. michiganense subsp. sepedonicum (C. michiganensis subsp. sepedonicus), was prepared by LiCl treatment and used as antigen to immunize Balb/c mice. The spleen cells from immunized mice were fused with myeloma cell line Sp2/o-Ag14. Six hybridoma cell lines (CH1-CH6) secreting monoclonal antibodies McAb1-McAb6 were established.

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