+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Production of plantlets from Aegle marmelos nucellar callus

, : Production of plantlets from Aegle marmelos nucellar callus. Plant Cell Reports 13(10): 570-573

Techniques have been developed for the regeneration of Aegle marmelos from nucellar explants. Slow-growing calli were induced from nucellar explants excised from 90-120 d-old developing fruits. The medium consisted of Murashige and Skoog formulation containing 40 g/l sucrose, 400 mg/l casein hydrolysate, 5 mg/l 1-naphthaleneacetic acid and 1 mg/l kinetin. The basal medium with high concentration (1-5 mg/l) of N6-benzyladenine (BA) and low concentration (0.1 mg/l) of NAA was suitable for regeneration of shoots from 3-month-old calli. Addition of 1 mg/l gibberellic acid (GA3) favoured shoot growth. Callus-derived shoots produced roots and developed into plantlets when transferred to half-strength MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l NAA. Approximately 5 months were required for the full regenerative process.

Accession: 002468062

PMID: 24196223

DOI: 10.1007/BF00234513

Download PDF Full Text: Production of plantlets from Aegle marmelos nucellar callus

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Islam, M.R.; Zaman, S.; Nasirujjaman, K., 2007: Regeneration of plantlets from node-derived callus in Aegle marmelos Corr. A protocol for the regeneration of Aegle marmelos from nodal explant was developed. For callus induction, nodal segments derived from in vitro grown shoots were cultured on Murashige and Skoog (MS) medium supplemented with different compositions o...

Arumugam, S.; Rao, M.V., 1996: In vitro production of plantlets from cotyledonary node cultures of Aegle marmelos (L.) Corr. A. marmelos is an important Ayurvedic medicinal tree species. Cotyledonary node explants, excised from 15-day-old seedlings of A. marmelos, were placed on Murashige and Skoog (MS) medium supplemented with BAP [benzyladenine], IBA, IAA or NAA. BAP...

Johri, B.M.; Ahuja, M.R., 1956: Development of endosperm and nucellar polyembryony in Aegle marmelos Correa. Other Rutaceae too show polyembryony, both nucellar and zygotic (from multiple gametophytes).

Hossain, M.; Karim, M.R.; Islam, R.; Joarder, O.I., 1993: Plant regeneration from nucellar tissues of Aegle marmelos through organogenesis. A protocol for organogenesis from nuclear explants excised from fertilized ovules of immature fruits of Aegle marmelos Corr. was developed. Adventitious buds were initiated on Murashige and Skoog's (MS) medium containing various combinations...

Bhati, R.; Shekhawat, N.S.; Arya, H.C., 1992: In vitro regeneration of plantlets from root segments of Aegle marmelos. Plantlets were regenerated from cultured root segments of A. marmelos, a fruit and forest tree. Root segments of plantlet cloned from mature tree, were cultured on Murashige and Skoog's (MS) medium containing various combinations of auxins an...

Hossain, M.; Islam, R.; Karim, M.R.; Joarder, O.I.; Biswas, B.K., 1994: Regeneration of plantlets from in vitro cultured cotyledons of Aegle marmelos Corr. (Rutaceae). Cotyledons from Aegle marmelos seedlings of various ages were cultured on MS medium supplemented with different combinations of growth regulators. The optimum seedling age was 10 days for shoot induction response and benzyladenine (BA) was superio...

Hazeena,M.S.; Sulekha,G.R., 2008: Callus induction and plantlet regeneration in Aegle marmelos Corr using cotyledon explants. A protocol was developed for callus induction and shoot regeneration from cotyledon explants of <i>Aegle marmelos</i> (L.) Corr., a medicinal tree. Murashige and Skoog (MS) medium supplemented with benzyl adenine (2.2 micro M) and 2,4-...

Bhardwaj, L.M.rillon, J.; Ramawat, K., 1995: Changes in the composition of membrane lipids in relation to differentiation in Aegle marmelos callus cultures. Changes in fatty acid, phospholipid and galactolipid contents during cellular and organ differentiation in Aegle marmelos have been described. Decrease in phosphatidylinositol content and presence of 3-trans-hexadecenoic acid in phosphatidylglycer...

Islam, R.; Hossain, M.; Karim, M.R.; Joarder, O.I., 1995: Regeneration of Aegle marmelos (L.) Corr. plants in vitro from callus cultures of embryonic tissues. Successful regeneration of plantlets from callus cultures derived from zygotic embryos is described for this multipurpose tree species. Details are given of the most appropriate culture media (Murashige and Skoog basal medium + various combination...

Prematilake, D.P.; Nilmini, H.A.S.; Kudagamage, C., 2006: Establishment of an in vitro plant regeneration system for Aegle marmelos (L.) Corr. via organogenic callus culture. A tissue-to-plant regeneration system was developed for Aegle marmelos using cotyledon tissues. This system was applicable to immature leaf and root tissues at lower efficiency. This procedure involved organogenic calluses formation on a MS-based...