+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Production of plantlets from Aegle marmelos nucellar callus

Plant Cell Reports 13(10): 570-573
Production of plantlets from Aegle marmelos nucellar callus
Techniques have been developed for the regeneration of Aegle marmelos from nucellar explants. Slow-growing calli were induced from nucellar explants excised from 90-120 d-old developing fruits. The medium consisted of Murashige and Skoog formulation containing 40 g/l sucrose, 400 mg/l casein hydrolysate, 5 mg/l 1-naphthaleneacetic acid and 1 mg/l kinetin. The basal medium with high concentration (1-5 mg/l) of N6-benzyladenine (BA) and low concentration (0.1 mg/l) of NAA was suitable for regeneration of shoots from 3-month-old calli. Addition of 1 mg/l gibberellic acid (GA3) favoured shoot growth. Callus-derived shoots produced roots and developed into plantlets when transferred to half-strength MS medium supplemented with 0.5 mg/l indole-3-butyric acid (IBA) and 0.5 mg/l NAA. Approximately 5 months were required for the full regenerative process.

Accession: 002468062

PMID: 24196223

DOI: 10.1007/BF00234513

Download PDF Full Text: Production of plantlets from Aegle marmelos nucellar callus

Related references

Regeneration of plantlets from node-derived callus in Aegle marmelos Corr. Biotechnology 6(1): 72-75, 2007

In vitro production of plantlets from cotyledonary node cultures of Aegle marmelos (L.) Corr. Advances in Plant Sciences 9(2): 181-186, 1996

Development of endosperm and nucellar polyembryony in Aegle marmelos Correa. Current Sci 25(5): 162-164, 1956

Plant regeneration from nucellar tissues of Aegle marmelos through organogenesis. Plant Cell Tissue & Organ Culture 34(2): 199-203, 1993

In vitro regeneration of plantlets from root segments of Aegle marmelos. Indian Journal of Experimental Biology 30(9): 844-845, 1992

Regeneration of plantlets from in vitro cultured cotyledons of Aegle marmelos Corr. (Rutaceae). Scientia Horticulturae 57(4): 315-321, 1994

Callus induction and plantlet regeneration in Aegle marmelos Corr using cotyledon explants. Journal Of Tropical Agriculture: 1 2, 79-84, 2008

Changes in the composition of membrane lipids in relation to differentiation in Aegle marmelos callus cultures. Plant cell tissue and organ culture 42(1): 33-37, 1995

Regeneration of Aegle marmelos (L.) Corr. plants in vitro from callus cultures of embryonic tissues. Current Science 69(6): 494-495, 1995

Establishment of an in vitro plant regeneration system for Aegle marmelos (L.) Corr. via organogenic callus culture. Ceylon Journal of Science, Biological Sciences 35(1): 87-90, 2006