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Production of somatic hybrids between Nicotiana benthamiana and N. tabacum and their resistance to aphids


, : Production of somatic hybrids between Nicotiana benthamiana and N. tabacum and their resistance to aphids. Plant Science Limerick 91(2): 213-222

In order to introduce aphid resistance into tobacco, somatic hybrids between N. tabacum and aphid resistant N. benthamiana were produced using protoplast fusion.

Accession: 002468119

DOI: 10.1016/0168-9452(93)90144-o

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Related references

Menczel L.; Nagy F.; Kiss Z.R.; Maliga P., 1981: Streptomycin resistant and sensitive somatic hybrids of nicotiana tabacum plus nicotiana knightiana correlation of resistance to nicotiana tabacum plastids. Protoplasts of N. tabacum SR1 (streptomycin resistant) and of N. knightiana (streptomycin sensitive) were fused using polyethylene glycol treatment. From 3 heterokaryons 500 clones were obtained. From the 43 investigated further, 6 resistant, 3 se...

Menczel, L.N.gy, F.K.ss, Z.; Maliga, P., 1981: Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to Nicotiana tabacum plastids. Theoretical and applied genetics9(3): 191-195

Menczel, L.; Nagy, F.; Kiss, Z.R.; Maliga, P., 1981: Streptomycin resistant and sensitive somatic hybrids of Nicotiana tabacum + Nicotiana knightiana: correlation of resistance to N. tabacum plastids. Protoplasts of N. tabacum SR1 (streptomycin resistant) and of N. knightiana (streptomycin sensitive) were fused using polyethylene glycol treatment. From three heterocaryons, 500 calluses were obtained. From the 43 which were further investigated,...

Nagao, T., 1982: Somatic hybridization by fusion of protoplasts 3. somatic hybrids of sexually incompatible combinations nicotiana tabacum + nicotiana repanda and nicotiana tabacum + salpiglossis sinuata. Somatic hybrid plants were regenerated following the fusion of mesophyll protoplasts of N. tabacum cv. Bright Yellow-Aurea (2n = 48) with those isolated from N. repanda (2n = 48), and with suspension culture protoplasts of S. sinuata (2n = 44). Th...

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Evans D.A.; Bravo J.E.; Kut S.A.; Flick C.E., 1983: Genetic behavior of somatic hybrids in the genus nicotiana nicotiana otophora plus nicotiana tabacum and nicotiana sylvestris plus nicotiana tabacum. Somatic hybrids were produced between N. tabacum and 2 closely related species in the genus Nicotiana, N. otophora and N. sylvestris, to evaluate interclonal variation and genetic behavior of these hybrids. As with the previously reported N. nesop...

Evans, D.; Bravo, J.1; Kut, S.1; Flick, C., 1983: Genetic behavior of somatic hybrids in the genus Nicotiana: Nicotiana otophora + Nicotiana tabacum and Nicotiana sylvestris + Nicotiana tabacum Protoplast fusion, genetic variability. Theoretical and applied genetics 65(2): 93-101

Toki S.; Kameya T., 1988: The use of streptomycin resistance and chlorophyll deficiency for selection of somatic hybrids between nicotiana tabacum and nicotiana rustica. To prove the usefulness for somatic hybridisation of a Nicotiana tabacum L. double mutant (SA), which is both streptomycin resistant and chlorophyll deficient, protoplasts obtained from SA suspension cultures were fused via the dextran method with...

Medgyesy P.; Menczel L.; Maliga P., 1980: The use of cytoplasmic streptomycin resistance chloroplast transfer from nicotiana tabacum into nicotiana sylvestris and isolation of their somatic hybrids. Leaf protoplasts of N. tabacum SR1 (2n = 4x = 48) treated with iodoacetate (10 mM; 25.degree. C; 30 min) and consequently unable to divide, and untreated leaf protoplasts of N. sylvestris (2n = 2x = 24) were fused using polyethylene glycol (PEG)....

Miller S.A.; Flick C.E.; Evans D.A., 1984: Inheritance of tobacco mosaic virus resistance in backcross progeny of somatic hybrids of nicotiana tabacum and nicotiana nesophila. Phytopathology 74(7): 851-852