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Production of the lethal acetylcholinesterase toxin by different Aeromonas hydrophila strains

, : Production of the lethal acetylcholinesterase toxin by different Aeromonas hydrophila strains. Journal of Fish Diseases 16(1): 73-78

Seven fish pathogenic isolates of Aeromonas hydrophila, one A. sobria and one A. caviae were investigated for productionof the fish lethal acetylcholinesterase toxin (AcChE-toxin). Western blotting was used for screening the ECP of these strains with a rabbit antiserum prepared against the purified toxin of strain B-32 and all the isolates (except A. sobria) gave positive results with different patterns of bands. The AcChE-toxin appears to be secreted as a protein of high molecular weight which is stable at -20 degree C, and in 90% of the strains tested, it appears to be split into lower molecular weight fragments by the action of other components presentin the ECP. The smallest, stable and highly active fragment has a MW of 15KDa.

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Accession: 002468142

DOI: 10.1111/j.1365-2761.1993.tb00849.x

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