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Production of zoospores, mode of infection, and inoculum potential of Pythium myriotylum propagules on cocoyam


, : Production of zoospores, mode of infection, and inoculum potential of Pythium myriotylum propagules on cocoyam. Journal of Phytopathology (Berlin) 140(1): 49-54

Zoospores of Pythium myriotylum were consistently produced from sporangia of detached mycelia in vitro in deionized water at pH 7.0 with 0.001 M sucrose in 24 h of continuous fluorescent light at 31 degree C. The oblong-to-pea shaped zoospores measured from 2.5-7.5 mu-m in diameter and remained motile for more than 48 h. The lengths of flagella were 1-1.5 times the diameter of the zoospores. Contaminated cultures of P. myriotylum were revived to pure isolates by the use of zoospores. The penetration of P. myriotylum propagules took from 6 to 7 h following contact of the inoculum with the roots, and the invasion was inter- and intracellular. At the minimum concentrations of 200 zoospores/ml or 180 mycelial strands/ml, P. myriotylum caused symptoms of CRRD within 3 to 6 days after inoculation of the roots of cocoyam plantlets, results indicating that the pathogen is very destructive in cocoyam.

Accession: 002468216

DOI: 10.1111/j.1439-0434.1994.tb00175.x

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