EurekaMag.com logo
+ Site Statistics
References:
54,215,046
Abstracts:
30,230,908
PMIDs:
28,215,208
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

RFLP analysis of highly polymorphic loci in barley



RFLP analysis of highly polymorphic loci in barley



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 85(8): 926-930



Barley middle-repeat sequences were screened for their ability to discriminate 51 barley commercial varieties. Two hordein clones, a clone encoding a leaf-specific thionin, a desiccation induced cDNA clone, a clone coding for 5S-rRNA and one corresponding to ubiquitin genes were tested. A very sensitive RFLP technique including four cutter restriction enzymes and denaturing 4% polyacrylamide gels were used to evidence the highest level of polymorphism.The RFLP data were analyzed by computer. Some probe/enzyme combinations were able to differentiate a large number of the cultivars tested, whereas three probe/enzyme combinations succeeded in identifying all the varieties. The use of this RFLP method can thus be suggested for cultivar identification in barley.

(PDF emailed within 0-6 h: $19.90)

Accession: 002472804

Download citation: RISBibTeXText

PMID: 24196141

DOI: 10.1007/BF00215030



Related references

An embryonal form of human myosin heavy chain complementary dna detects a highly polymorphic rflp loci. Cytogenetics & Cell Genetics 51(1-4): 1080, 1989

Genetic analysis of the obligate parasitic barley powdery mildew fungus based on RFLP and virulence loci. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 79(5): 705-712, 1990

Two new highly polymorphic microsatellite loci and inadvertent minisatellite loci for Lymnaea auricularia. Journal of Molluscan Studies. February; 701: 95-96, 2004

An exact test for the association between the disease and alleles at highly polymorphic loci with particular interest in the haplotype analysis. Biometrics 57(3): 9-78, 2001

Simple approach to reduce PCR artefact formation leads to reliable genotyping of MHC and other highly polymorphic loci--implications for evolutionary analysis. Gene 427(1-2): 117-123, 2008

Highly polymorphic RFLP probes as diagnostic tools. Cold Spring Harbor Symposia on Quantitative Biology 51 Pt 1: 317-324, 1986

Screening of highly-polymorphic RFLP probes in Oryza sativa L. Journal of Genetics & Breeding 52(1): 39-48, March, 1998

Cultivar identification in T. aestivum using highly polymorphic RFLP probes. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 86(7): 833-836, 1993

Cultivar identification in Triticum aestivum using highly polymorphic RFLP probes. Theoretical & Applied Genetics 86(7): 833-836, 1993

Highly polymorphic RFLP marker D4S130 (CRI-L518) maps to distal 4q. Nucleic Acids Research 19(20): 5802, 1991

A highly polymorphic PCR/RFLP marker in the canine type II collagen gene (COL2A1). Animal Genetics 29(5): 407-408, 1998

Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 89(5): 606-614, 1994

A highly polymorphic RFLP marker in the canine transduction alpha -1 subunit gene (GNAT1). Animal Genetics 27(5): 372-373, 1996