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RFLP analysis of opaque2 genotypes in segregating populations

RFLP analysis of opaque2 genotypes in segregating populations

Maize Genetics Cooperation Newsletter ( 67): 26-27

As part of a breeding programme to produce maize lines with hard, corneous endosperm, an RFLP assay was developed to identify polymorphisms tightly linked to the o2 locus. The procedures were field tested by crossing Tx5855 to plants from 4 hard endosperm o2/o2 populations (POP20 to POP23) from South Africa and 2 CIMMYT Quality Protein Maize (QPM) populations (POB67 and POB68). DNA was isolated from leaf samples taken from the F1 and BC1 and analysed using RFLPs.

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Accession: 002472807

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