+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination



Recombination walking: genetic selection of clones from pooled libraries of yeast artificial chromosomes by homologous recombination



Proceedings of the National Academy of Sciences of the United States of America 90(17): 8118-8122



A genetic selection approach based on homologous recombination is described. Called "recombination walking," the approach is grounded in the genetic selection of specific human clones from a yeast artificial chromosome library. It is a system designed to allow multiple walking steps to proceed in parallel. The screening method requires neither filter hybridization or DNA sequencing of individual clones nor the synthesis of the unique primers required for PCR techniques but is based on the specificity of genetic recombination in yeast. This system represents an efficient approach to library screening and is also suitable for the chromosome-walking approaches employed in the identification of genes associated with common diseases. .

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002475117

Download citation: RISBibTeXText

PMID: 8367472

DOI: 10.2307/2362976


Related references

Method for homologous-recombination screening of recombinant-DNA clones in yeast host libraries. Official Gazette of the United States Patent & Trademark Office Patents 1212(3): 2818, 1998

Rescue of end fragments of yeast artificial chromosomes by homologous recombination in yeast. Nucleic Acids Research 19(18): 4943-4948, 1991

Genetic change of recombination value in Drosophila melanogaster. I. Artificial selection for high and low recombination and some properties of recombination-modifying genes. Genetics 70(3): 419-432, 1972

Detection of homologous recombination between yeast artificial chromosomes with overlapping inserts. Nucleic Acids Research 19(5): 997, 1991

Introduction of yeast artificial chromosomes into embryonic stem cells via homologous recombination. American Journal of Human Genetics 59(4 Suppl. ): A365, 1996

Coupling homologous recombination with growth selection in yeast: a tool for construction of random Dna sequence libraries. BioTechniques 37(2): 202-206, 2004

Coupling homologous recombination with growth selection in yeast: a tool for construction of random DNA sequence libraries. Biotechniques 37(2): 202 204 206, 2004

Improved genetic selection for screening bacteriophage libraries by homologous recombination in vivo. Proceedings of the National Academy of Sciences of the United States of America 87(8): 3166-3169, 1990

Incorporation of copy-number control elements into yeast artificial chromosomes by targeted homologous recombination. Mammalian Genome. 4(3): 141-147, 1993

Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector. Journal of Biotechnology 139(3): 211-213, 2009

Isolation of specific clones from nonarrayed BAC libraries through homologous recombination. Journal of Biomedicine and Biotechnology 2011: 560124, 2011

Homologous recombination using bacterial artificial chromosomes. Cold Spring Harbor Protocols 2015(2): 180-190, 2015

Homologous Recombination between Episomal Plasmids and Chromosomes in Yeast. Genetics 105(4): 843-856, 1983

Large-insert BAC/YAC libraries for selective re-isolation of genomic regions by homologous recombination in yeast. Genomics 77(1-2): 27-34, 2001

Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display. Nucleic Acids Research 32(3): E36, 2004