EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Regeneration of plants from cultured guard cell protoplasts of Nicotiana glauca (Graham)


Regeneration of plants from cultured guard cell protoplasts of Nicotiana glauca (Graham)



Plant Science Limerick 97(2): 199-208



DOI: 10.1016/0168-9452(94)90057-4

Guard cell protoplasts were isolated from adaxial epidermal leaf tissue of plants grown under fluorescent light (800-900 micro mol/msuperscript 2 per s photons of photosynthetically active radiation). To increase the probability that guard cells were of uniform osmotic potential, leaves were collected in darkness, just before the beginning of each light period.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002476337

Download citation: RISBibTeXText

Related references

Regeneration of plants from cultured guard cell protoplasts of Nicotiana glauca. Plant Physiology (Rockville) 102(1 Suppl. ): 31, 1993

Division of guard cell protoplasts of nicotiana glauca graham in liquid cultures. Plant Cell and Environment 14(7): 691-698, 1991

Effects of temperature and abscisic acid on survival and differentiation of cultured guard cell protoplasts of Nicotiana glauca. Plant Physiology (Rockville) 105(1 Suppl. ): 121, 1994

Cultured guard cell protoplasts of Nicotiana glauca retain the capacity for blue light-induced swelling. Plant Physiology (Rockville) 114(3 Suppl. ): 281, 1997

Approaches to evaluating the extent to which guard cell protoplasts of Nicotiana glauca (tree tobacco) retain their characteristics when cultured under conditions that affect their survival, growth, and differentiation. Journal of Experimental Botany 49(Special issue): 377-386, 1998

Regeneration of plants from cultured guard cell protoplasts. Official Gazette of the United States Patent and Trademark Office Patents 1185(3): 1931, 1996

Analysis of Guard Cell Viability and Action in Senescing Leaves of Nicotiana glauca (Graham), Tree Tobacco. Plant Physiology 79(1): 7-10, 1985

Responses of guard cell protoplasts of Nicotiana glauca from leaves with closed or open stomata to red and blue light. Plant Physiology 89(Suppl. 4): 23, 1989

Potassium chloride induced dark swelling of guard cell protoplasts from nonsenescing or senescing leaves of nicotiana glauca. Plant Physiology 86(4 Suppl.): 41, 1988

Comparison of physiological responses of guard cell protoplasts of Nicotiana glauca isolated from leaves collected before dawn or at midday. Physiologia Plantarum 86(2): 221-230, 1992

Somatic hybrids of plants by fusion of protoplasts observations on the model system nicotiana glauca nicotiana langsdorffii hybrid parent. Molecular and General Genetics 165(3): 239-246, 1978

Guard cell protoplasts. Isolation, culture, and regeneration of plants. Methods in Molecular Biology 111: 243-257, 1999

Guard cell protoplasts: isolation, culture, and regeneration of plants. Methods in Molecular Biology 318: 233-252, 2006

Somatic hybridization between nicotiana rustica and nicotiana tabacum 1. isolation and culture of protoplasts and regeneration of plants from cell cultures of wild type and chlorophyll deficient strains. Canadian Journal of Botany 59(2): 208-219, 1981

Interspecific transfer of only part of genome by fusion between nonirradiated protoplasts of nicotiana glauca and x ray irradiated protoplasts of nicotiana langsdorffii. Japanese Journal of Genetics 58(6): 545-554, 1983