Regeneration of plants from cultured guard cell protoplasts of Nicotiana glauca (Graham)
Regeneration of plants from cultured guard cell protoplasts of Nicotiana glauca (Graham)
Sahgal, P.; Martinez, G.V.; Roberts, C.; Tallman, G.
Plant Science Limerick 97(2): 199-208
1994
DOI: 10.1016/0168-9452(94)90057-4
Guard cell protoplasts were isolated from adaxial epidermal leaf tissue of plants grown under fluorescent light (800-900 micro mol/msuperscript 2 per s photons of photosynthetically active radiation). To increase the probability that guard cells were of uniform osmotic potential, leaves were collected in darkness, just before the beginning of each light period.