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Regulation of insulin-like growth factor binding protein secretion by a murine mammary epithelial cell line


, : Regulation of insulin-like growth factor binding protein secretion by a murine mammary epithelial cell line. Experimental Cell Research 209(2): 183-188

The regulation of insulin-like growth factor binding protein (IGFBP) secretion by mammogenic and lactogenic hormones was investigated in a mouse mammary epithelial cell line (COMMA-D/MME). Cells were grown to confluency on plastic culture plates in serum-containing medium. The confluent cells were exposed to the hormonal treatments in serum-free medium for 6 days. Conditioned medium was collected on Days 5 and 6 and analyzed for IGFBPs by 125I-labeled insulin-like growth factor (IGF)-II ligand blotting and quantified by densitometry. IGFBP data were expressed as absorbance units x millimeters (AUxmm) that were corrected for DNA per well. In basal serum-free media, the COMMA-D/MME cells secreted predominantly IGFBP-3, but also some IGFBP-2. The mammogenic growth factors, IGF-I (13.3 nM) and epidermal growth factor (EGF; 1.7 nM), both stimulated DNA synthesis (P < 0.05); however, their effects on IGFBP-3 secretion differed. IGF-I stimulated IGFBP-3 secretion whereas EGF was inhibitory. In addition, EGF inhibited IGFBP-2 secretion and IGF-I tended to increase it. No interaction was observed between IGF-I and EGF for any of the parameters measured. Three lactogenic hormones (insulin, 154 nM; prolactin, 4.3 nM; and cortisol, 1.4 microM) in all combinations were tested to determine their effects on IGFBP secretion. Insulin stimulated IGFBP-3 secretion and DNA synthesis, but had no effect on IGFBP-2 secretion. Cortisol inhibited IGFBP-3 secretion and DNA synthesis, but increased IGFBP-2 secretion. Prolactin had no effect on any of the parameters examined. In summary, the COMMA-D/MME cells secrete IGFBP-2 and IGFBP-3 in serum-free media. Although the secretion of IGFBP-2 and IGFBP-3 in serum-free media. Although the secretion of IGFBP-2 and IGFBP-3 was regulated by several of the hormones and growth factors tested, no clear distinction was observed between the mammogenic and lactogenic treatments.

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Accession: 002477157

PMID: 7505235

DOI: 10.1006/excr.1993.1300

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Duncan W.J.; Freedom R.M.; Olley P.M.; Rowe R.D., 1981: 2 dimensional echo cardiographic identification of hemitruncus anomalous origin of 1 pulmonary artery from the ascending aorta with the other pulmonary artery arising normally from the right ventricle. Two-dimensional echocardiography (2DE) was performed on a 2300-g human newborn who presented with congestive heart failure. Clinical examination suggested left-to-right shunt with pulmonary hypertension. Cardiac catheterization and angiography dem...

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Granfors K.; Toivanen A., 1986: Immunoglobulin a anti yersinia antibodies in yersinia triggered reactive arthritis. Patients who develop reactive arthritis after yersinia enteritis are characterised by high and persisting IgA-anti-yersinia antibodies. We have further analysed the humoral immune response to yersinia in this condition. Total concentrations of IgM...

Guimaraes,J.L.; Wypych,F.; Saul,C.K.; Ramos,L.P.; Satyanarayana,K.G., 2010: Studies of the processing and characterization of corn starch and its composites with banana and sugarcane fibers from Brazil. This paper presents results on the characterization of corn starch by X-ray powder diffraction and thermal analysis, as well as processing and characterization of starch-banana/sugarcane bagasse fiber composites. X-ray diffraction studies revealed...

Massey C.D., 1991: Conducting a site selection study for a non reactor nuclear facility within doe site boundaries. Health Physics 60(SUPPL 2): S68

Philippart, A.I.; Sarnaik, A.P.; Belenky, W.M., 1980: Respiratory support in pediatric surgery. Surgical Clinics of North America 60(6): 1519-1532

Amschler, D.H., 2002: The alarming increase of type 2 diabetes in children. Journal of School Health 72(1): 39-41

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