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Regulation of laccase biosynthesis in the plant-pathogenic fungus Cryphonectria parasitica by double-stranded RNA


, : Regulation of laccase biosynthesis in the plant-pathogenic fungus Cryphonectria parasitica by double-stranded RNA. Journal of Bacteriology 173(24): 8000-8003

Transmissible hypovirulence of the chestnut blight fungus, Cryphonectria parasitica, is associated with cytoplasmic double-stranded-RNA (dsRNA) viruses. The fungal laccase has attracted interest because its activity is reduced in hypovirulent dsRNA-containing strains. A laccase cDNA clone was isolated by screening a cDNA expression library with antibodies against the purified extracellular laccase. The amino acid sequence deduced from part of the cDNA clone revealed high homology to other fungal laccases, especially to the Neurospora crassa laccase. A major laccase transcript 2.3 kb in length was detected in Northern (RNA) blots. In liquid culture, extracellular laccase activity was reduced by about 75% in the hypovirulent (dsRNA-free) strain EP155/2. In contrast, production of biomass was not affected by the dsRNA. Northern blot analysis indicated that dsRNA down regulates laccase biosynthesis by reducing laccase mRNA accumulation. The laccase gene is one of several developmentally, regulated genes affected by the presence of dsRNA.


Accession: 002477163

PMID: 1744058

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Related references

Rigling D.; Van Alfen N.K., 1991: Regulation of laccase biosynthesis of the chestnut blight fungus cryphonectria parasitica by double stranded rna dsrna. Phytopathology 81(10): 1170

Rigling D.; Heiniger U.; Hohl H.R., 1989: Reduction of laccase activity in double stranded rna containing hypovirulent strains of cryphonectria parasitica. Double-stranded RNA (dsRNA) was detected in hypovirulent but not in virulent strains of Cryphonectria (Endothia) parasitica isolated in Switzerland. To test for phenol oxidase activity we grew the strains on malt extract agar containing tannic aci...

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Peever, T.L.; Wang, K.; Milgroom, M.G., 1996: Genetic diversity of double-stranded RNAs infecting the chestnut blight fungus, Cryphonectria parasitica, in China and Japan. Phytopathology 86(11 SUPPL ): S90

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Varley, D.A.; Podila, G.K.; Hiremath, S.T., 1992: Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs. Plant-pathogenic fungi produce cutinase, an enzyme required to degrade plant cuticles and facilitate penetration into the host. The absence of cutinase or a decrease in its production has been associated with a decrease in pathogenicity of the fun...