+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of Mitochondrial Function and Biogenesis in Cucumber (Cucumis sativus L.) Cotyledons during Early Seedling Growth

Plant Physiology 99(1): 60-66
Regulation of Mitochondrial Function and Biogenesis in Cucumber (Cucumis sativus L.) Cotyledons during Early Seedling Growth
The aim of this work was to characterize the respiratory metabolism of the greening cotyledons of cucumber (Cucumis sativus L.) during early seedling growth and to investigate how this is integrated with changes in mitochondrial biogenesis and function. In light-grown cotyledons, lipid mobilization extended from germination to 6 days postimbibition, reaching a maximum at 3 to 4 days postimbibition. The rate of dark oxygen uptake reached a maximum at 2 days postimbibition in dark-grown and 3 days postimbibition in light-grown cotyledons. Development of photosynthetic capacity occurred from 4 to 7 days postimbibition. In dark-grown cotyledons, lipid mobilization extended beyond 7 days postimbibition, and there was no greening or acquisition of photosynthetic competence. Measurements of mitochondrial function indicated that the respiratory capacity of the tissue changed such that during lipid mobilization there was a much greater capacity for the operation of the nondecarboxylating portion of the citric acid cycle (succinate to oxaloacetate), whereas during the development of photosynthetic function the activity of the remainder of the cycle (oxaloacetate to succinate) was induced. Comparison of the maximum capacities for mitochondrial substrate oxidations in vitro with the rates of in vivo substrate oxidations, predicted from the rate of lipid breakdown, indicated that mitochondria in this tissue operate at or below state 4 rates, suggesting limitation by both availability of ADP and substrate.

Accession: 002477182

PMID: 16668884

DOI: 10.2307/4274303

Related references

Regeneration of plants from callus tissue of cucumber cucumis sativus seedling cotyledons. Plant Physiology (Rockville) 75(SUPPL 1): 15, 1984

Regeneration of plants from callus tissue of cucumber (Cucumis sativus L.) seedling cotyledons. Plant Physiology 75(1, Supplement): 15, 1984

Chloroplast biogenesis. 38. Quantitative detection of a chlorophyllide b pool in higher plants Cucumber cotyledons, Cucumis sativus. Biochimica et biophysica acta: bioenergetics, 679(2): 248-260, 1982

Changes in phosphatase activity during germination and in early phases of cucumber cucumis sativus seedling growth. Acta Societatis Botanicorum Poloniae 49(1-2): 91-104, 1980

Dark-chilling induces substantial structural changes and modifies galactolipid and carotenoid composition during chloroplast biogenesis in cucumber (Cucumis sativus L.) cotyledons. Plant Physiology and Biochemistry 111(): 107-118, 2016

Cyto kinin induced wall extensibility in excised cotyledons of radish raphanus sativus cultivar early scarlet globe and cucumber cucumis sativus cultivar marketer. Plant Physiology (Rockville) 68(1): 107-110, 1981

Inhibition of zeatin induced growth of cucumber cucumis sativus cotyledons by sulfite ions. Plant & Cell Physiology 26(1): 123-130, 1985

Kinetin promotion of expansion growth and the rna synthesis in cucumber cucumis sativus cultivar monastyrski cotyledons. Acta Physiologiae Plantarum 6(4): 173-180, 1984

Evaluation of H+ hydrogen ion secretion relative to zeatin-induced growth of detached cucumber cotyledons Cucumis sativus. 1982

Relationship of beta glucosidase activity to the growth of cucumber cucumis sativus cultivar chipper cotyledons and fruits. Hortscience 10(2): 147, 1975