+ Translate

Regulation of nodule efficiency by the undisturbed soybean plant

, : Regulation of nodule efficiency by the undisturbed soybean plant. Journal of Experimental Botany 42(238): 687-691

Acetylene reduction activity (ARA) has been widely used to estimate comparative rates of nitrogen fixation in soybean [Glycine max (L.) Merr.]. To use the non-destructive closed-container ARA assay to study plant-mediated regulation of N2 fixation, it was first necessary to determine the validity of the ARA procedure. Thus, well-nodulated, hydroponically-grown soybean plants were individually assayed for ARA twice weekly for 5 weeks. Plants were subsequently analysed for total nitrogen by the Kjeldahl procedure and the values obtained were compared with the N-accumulation values estimated by ARA. For the 168 plants examined, ARA measurements underestimated N2 fixation by approximately 50%. However, N2 fixation was underestimated by approximately 80% in plants with a very low ARA whereas N2 fixation was underestimated by only 20% in plants with a high ARA. Because of the accuracy and reliability of the Kjeldahl meaurements, these reuslts show that modulated soybean plants can exhibit different efficiencies in acetylene reduction. Thus, vigorous, well-nodulated, hydroponically-grown soybean plants readily control nodule efficiency and the N fixation process.

(PDF 0-2 workdays service)

Accession: 002477196

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Krikunets V.M., 1990: Response of soybean to small doses of fertilizer nitrogen depending on the efficiency of plant symbiosis with nodule bacteria. Soybean response to small doses of fertilizer nitrogen applied before sowing is established to depend on a degree of inoculation of plants (the symbiotic effectiveness under the zero dose of the element). In the case of plant unsymbiotic state the...

Parsons R.; Day D.A., 1990: Structure of the soybean nodule cortex in relation to oxygen diffusion and regulation of nodule activity. Gresshoff, P M , Et Al (Ed ) Nitrogen Fixation: Achievements And Objectives; 8th International Congress on Nitrogen Fixation, Knoxville, Tennessee, Usa, May 20-26, Xix+869p Routledge, Chapman And Hall: New York, New York, Usa; London, England, Uk Illus 360

Bhuvaneswari, T.V.; Lesniak, A.P.; Bauer, W.D., 1988: Efficiency of nodule initiation in cowpea and soybean. When serial dilutions of a suspension of Bradyrhizobium japonicum strain 138 were inoculated onto both soybean [Glycine max] and cowpea [Vigna unguiculata] roots, the formation of nodules in the initially susceptible region of the roots of both ho...

Milic, V.; Mrkovacki, N.; Popovic, M.; Malencic,, 2002: Nodule efficiency of three soybean genotypes inoculated by different methods. The objective of the study was to investigate how the inoculation of soyabean seed (cv. Afrodita and lines NS-L-2016 and NS-L-300168) with strains of Bradyrhizobium japonicum (1, 1a, and 2b), Azotobacter chroococcum (3, 13, and 14), and GA3 (gibbe...

Riede, W.; Bucherer, H., 1939: On longevity, activity and efficiency in soybean nodule bacteria. soyabean root nodule bacteria, which had been preserved in a dried condition for fifteen years, were found to be still viable.

Rahman, H.H.; Arima, Y.; Watanabe, K.; Sekimoto, H., 1999: Adequate range of boron nutrition is more restricted for root nodule development than for plant growth in young soybean plant. A pot experiment was conducted under growth chamber conditions to determine the lower and upper critical levels of boron (B) for plant growth, nodule development, and nodule acetylene reduction activity (ARA) in young soyabean plants. Plants of a...

Caetano-Anollés, G.; Gresshoff, P.M., 1991: Efficiency of nodule initiation and autoregulatory responses in a supernodulating soybean mutant. We compared the formation of nodules on the primary roots of a soybean cultivar (Glycine max (L.) Merr. cv. Bragg) and a supernodulating mutant derivative, nts382. Inoculation with Bradyrhizobium japonicum USDA 110 at different times after seed im...

Walsh, K.B.; Thorpe, M.R.; Minchin, P.E.H., 1998: Photoassimilate partitioning in nodulated soybean. III. The effect of changes in nodule activity shows that carbon supply to the nodule is not linked to nodule nitrogen metabolism. The hypothesis that photoassimilate partitioning to the soybean nodule is controlled by the rate of N export (via an effect on the turgor of the unloading pathway) was tested. Cessation of N2 fixation due to exposure of the nodulated root to Ar:O2...

T.J.C.; Buttery B.R., 1988: Soil compaction reduces nodulation nodule efficiency and growth of soybean and white bean. The effect of soil compaction on nodulation, nodule efficiency, and growth of soybean [Glycine max (L.) Merr.] and white bean (Phaseolus vulgaris L.) was investigated in a controlled environment. There was an inverse relationship between soil comp...

T.J.C.; Ford R.E.; Grau C.R., 1970: Some factors affecting the nodulation and nodule efficiency in soy beans d infected by soybean mosaic virus. Phytopathology 60(11): 1653-1656