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Regulation of phase-specific genes in the more general switching system of Candida albicans strain 3153A

Journal of Medical and Veterinary Mycology 32(4): 287-294

Regulation of phase-specific genes in the more general switching system of Candida albicans strain 3153A

It was previously demonstrated that the genes PEP1 and Op4 are selectively transcribed in the opaque phase of the white-opaque transition in Candida albicans strain WO-1, and that the gene Wh11 is selectively transcribed in the white phase. However, the white-opaque transition occurs in only a minority of C. albicans strains and the opaque cell phenotype is highly specialized. To test whether these genes are regulated in more mainstream switching systems in C. albicans, their expression was analysed in the switching system of the laboratory strain 3153A. Both PEP1 and Op4 are not expressed in the basic smooth phenotype of 3153A, but they are expressed in switch phenotypes. However, expression is dissociated. Op4 is expressed in irregular wrinkle, star and revertant-smooth cells, while PEP1 is expressed only in star cells. Wh11 is expressed in the basic smooth and three tested variant phenotypes and is again associated with growth in a round budding form. The results support the general conclusion that PEP1 and Op4 are under the strict regulation of switching in C. albicans.

Accession: 002477217

PMID: 7983573

DOI: 10.1080/02681219480000361

Related references

Ramsey, H.; Morrow, B.; Soll, D.R., 1994: An increase in switching frequency correlates with an increase in recombination of the ribosomal chromosomes of Candida albicans strain 3153A. When cells of Candida albicans strain 3153A are treated with a low dose of ultraviolet irradiation, they switch at high frequency (10(-2)) between a number of switch phenotypes discriminated by colony morphology. Clones from switching lineages exh...

Morrow, B.; Srikantha, T.; Anderson, J.; Soll, D.R., 1993: Coordinate regulation of two opaque-phase-specific genes during white-opaque switching in Candida albicans. Cells of Candida albicans WO-1 switch spontaneously and frequently between a white and an opaque CFU. Recently, an opaque-phase-specific cDNA, PEP1, was cloned and was demonstrated to code for a pepsinogen. By using a differential hybridization sc...

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