+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of plant nodule-specific genes expressed in alfalfa nodules arrested at an early stage of development

, : Regulation of plant nodule-specific genes expressed in alfalfa nodules arrested at an early stage of development. Plant Science (Limerick) 101(1): 65-73

We are investigating plant nodule-specific gene regulation in symbiotic nitrogen fixing root nodules. The strategy that we have used to obtain nodulin genes involved in the formation of the nodule organ structure is described. This strategy is particularly well suited for use with alfalfa, because of the strength of the genetics of its microbial symbiont, Rhizobium meliloti. Genes obtained using this strategy are those that are the indirect or direct targets of the developmental pathway set in motion by nodulation signal transduction, although it is impossible to predict whether the genes obtained will be activated early or late, in the temporal sense, during nodule development. Further results on the newly described nodulin ENOD8 are described, including the sequence of a new allele of ENOD8. ENOD8's polypeptide has similarity to the APG polypeptides of Arabidopsis thaliana and Brassica napus. Because we hypothesized that ENOD8 might be the functional equivalent of APG in alfalfa and because the APG genes are expressed in developing anthers, we assayed for ENOD8 expression n flowers and flower buds of alfalfa. We detected ENOD8 expression only in nodules.

Accession: 002477231

DOI: 10.1016/0168-9452(94)90165-1

Download PDF Full Text: Regulation of plant nodule-specific genes expressed in alfalfa nodules arrested at an early stage of development

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Dickstein, R.; Bisseling, T.; Reinhold, V.N.; Ausubel, F.M., 1988: Expression of nodule-specific genes in alfalfa root nodules blocked at an early stage of development. To help dissect the molecular basis of the Rhizobium-legume symbiosis, we used in vitro translation and Northern blot analysis of nodule RNA to examine alfalfa-specific genes (nodulins) expressed in two types of developmentally defective root nodu...

Dickstein, R.; Prusty, R.; Peng, T.; Ngo, W.; Smith, M.E., 1993: ENOD8, a novel early nodule-specific gene, is expressed in empty alfalfa nodules. The alfalfa ENOD8 nodule-specific gene is expressed in empty nodules elicited by exopolysaccharide-deficient Rhizobium meliloti, and it is expressed early in nodule development. An ENOD8 cDNA was sequenced and found to encode a novel product. Its...

Dunn, K.; Dickstein, R.; Feinbaum, R.; Burnett, B.K.; Peterman, T.K.; Thoidis, G.; Goodman, H.M.; Ausubel, F.M., 1988: Developmental regulation of nodule-specific genes in alfalfa root nodules. We have cloned alfalfa nodule-specific cDNAs that code for leghemoglobin (Lb), glutamine synthetase (GS), and three unidentified nodulins. Hybrid-select translation of nodule RNA followed by 2-D gel electrophoresis showed that the Lb-specific cDNA...

Dickstein R.; Prabhu N.; Prusty R., 1992: Plant nodule specific genes expressed in empty medicago nodules elicited by rhizobium meliloti exopolysaccharide mutants. Plant Physiology (Rockville) 99(1 SUPPL): 110

Dickstein, R.; Scheirer, D.C.; Fowle, W.H.; Ausubel, F.M., 1991: Nodules elicited by Rhizobium meliloti heme mutants are arrested at an early stage of development. Heme-deficient mutants of Rhizobium and Bradyrhizobium have been found to exhibit diverse phenotypes with respect to symbiotic interactions with plant hosts. We observed that R. meliloti hemA mutants elicit nodules that do not contain intracellula...

Norris, J.H.; Macol, L.A.; Hirsch, A.M., 1988: Nodulin gene expression in effective alfalfa nodules and in nodules arrested at three different stages of development. Nodulin gene expression was analyzed in effective and ineffective root nodules of alfalfa (Medicago sativa L. cv Iroquois) elicited by three different Rhizobium meliloti mutants: an exoB mutant having defective acidic exopolysaccharide that does n...

Ribeiro, A.; Akkermans, A.D.; van Kammen, A.; Bisseling, T.; Pawlowski, K., 1995: A nodule-specific gene encoding a subtilisin-like protease is expressed in early stages of actinorhizal nodule development. To identify genes specifically expressed during early stages of actinorhizal nodule development, a cDNA library made from poly(A) RNA from root nodules of Alnus glutinosa was screened differentially with nodule and root cDNA, respectively. Seven n...

Lang-Unnasch, N.; Ausubel, F.M., 1985: Nodule-specific polypeptides from effective alfalfa root nodules and from ineffective nodules lacking nitrogenase. At least 9 nodule-specific polypeptides, in addition to leghaemoglobin, were immunologically identified in Medicago sativa nodules formed with Rhizobium meliloti. One or more of these polypeptides was antigenically related to nodule-specific polyp...

Pawlowski, K.; Twigg, P.; Dobritsa, S.; Guan, C.; Mullin, B.C., 1997: A nodule-specific gene family from Alnus glutinosa encodes glycine- and histidine-rich proteins expressed in the early stages of actinorhizal nodule development. Two cDNAs representing different members (agNt84 and ag164) of a gene family encoding glycine- and histidine-rich proteins have been isolated from cDNA libraries from Alnus glutinosa root nodules. Expression of the corresponding genes could only b...

Lang Unnasch N.; Ausubel F.M., 1985: Nodule specific polypeptides from effective alfalfa medicago sativa root nodules and from ineffective nodules lacking nitrogenase. In addition to leghemoglobin, at least 9 nodule-specific polypeptides from the alfalfa Rhizobium meliloti symbiosis were identified by immune assay. Some of these polypeptides may be subunits of large proteins but none appeared to be subunits of t...