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Regulation of plant nodule-specific genes expressed in alfalfa nodules arrested at an early stage of development


, : Regulation of plant nodule-specific genes expressed in alfalfa nodules arrested at an early stage of development. Plant Science (Limerick) 101(1): 65-73

We are investigating plant nodule-specific gene regulation in symbiotic nitrogen fixing root nodules. The strategy that we have used to obtain nodulin genes involved in the formation of the nodule organ structure is described. This strategy is particularly well suited for use with alfalfa, because of the strength of the genetics of its microbial symbiont, Rhizobium meliloti. Genes obtained using this strategy are those that are the indirect or direct targets of the developmental pathway set in motion by nodulation signal transduction, although it is impossible to predict whether the genes obtained will be activated early or late, in the temporal sense, during nodule development. Further results on the newly described nodulin ENOD8 are described, including the sequence of a new allele of ENOD8. ENOD8's polypeptide has similarity to the APG polypeptides of Arabidopsis thaliana and Brassica napus. Because we hypothesized that ENOD8 might be the functional equivalent of APG in alfalfa and because the APG genes are expressed in developing anthers, we assayed for ENOD8 expression n flowers and flower buds of alfalfa. We detected ENOD8 expression only in nodules.

Accession: 002477231

DOI: 10.1016/0168-9452(94)90165-1

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Related references

Dickstein, R.; Bisseling, T.; Reinhold, V.N.; Ausubel, F.M., 1988: Expression of nodule-specific genes in alfalfa root nodules blocked at an early stage of development. To help dissect the molecular basis of the Rhizobium-legume symbiosis, we used in vitro translation and Northern blot analysis of nodule RNA to examine alfalfa-specific genes (nodulins) expressed in two types of developmentally defective root nodu...

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