+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of Plastid Gene Expression during Photooxidative Stress

, : Regulation of Plastid Gene Expression during Photooxidative Stress. Plant Physiology 99(4): 1406-1415

We have used the carotenoid biosynthesis inhibitor norflurazon to study the relationship between chloroplast and nuclear gene expression and the mechanisms by which plastid mRNA accumulation is regulated in response to photooxidative stress. By treating 4-week-old hydroponic spinach plants (Spinacea oleracea), we were able to determine the response at two distinct stages of chloroplast development. For all parameters studied, differences were found between the norflurazon-treated young and mature leaves. Young leaves lost essentially all pigment content in the presence of norflurazon, whereas mature leaves retained more than 60% of their chlorophyll and carotenoids. The accumulation of plastid mRNA was determined for several genes, and we found a decrease in mRNA levels for all genes except psbA in herbicide-treated young leaves. For genes such as atpB, psbB, and psaA, there was a corresponding change in the relative level of transcription, but for psbA and rbcL, transcription and mRNA accumulation were uncoupled. In norflurazon-treated mature leaves, all plastid mRNAs except psaA accumulated to normal levels, and transcription levels were either normal or higher than corresponding controls. This led to the conclusion that plastid mRNA accumulation is regulated both transcriptionally and posttranscriptionally in response to photooxidative stress. Although direct photooxidative damage is confined to the plastid and peroxisome, there is a feedback of information controlling the transcription of nuclear-encoded plastid proteins. Considerable evidence has accumulated implicating a "plastid factor" in this control. Therefore, the expression of several nuclear-encoded plastid proteins and the corresponding mRNAs were determined. Although the levels of both the small subunit of ribulose-1,5-bisphosphate carboxylase and the light harvesting chlorophyll a/b-binding protein and corresponding mRNAs were reduced, a 28-kilodalton chloroplast RNA-binding protein and corresponding mRNA were at normal levels in norflurazon-treated plants. Changes in mRNA and protein levels were not the result of a general loss due to photooxidation but rather the result of selective stabilization of certain components. The response of both genomes to photooxidative stress is discussed in terms of the postulated plastid factor.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002477235

PMID: 16669052

DOI: 10.1104/pp.99.4.1406

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Hansen, J.E., 1967: Pseudocysts of the auricle in Caucasians. A clinical problem in which a spontaneous collection of fluid located within the cartilage of the auricle in healthy white men is presented. Six clinical cases are reviewed in which patients responded to surgical therapy without deformity of the a...

Klein, G. C.; Baker, C. N.; Jones, W. L., 1971: Upper limits of normal anti strepto lysin o and anti dnase b titers. Applied Microbiology 21(6): 999-1001

Fabian, Ekkehard, 2002: Injector systems for foldable intraocular lens implantation. Kohnen, Thomas [Editor] Developments in Ophthalmology Modern cataract surgery 147-154

Smith, E. G.; Janzen, H. H.; Newlands, N. K., 2007: Energy balances of biodiesel production from soybean and canola in Canada. Biodiesel is currently produced in Canada mostly from recycled oils and animal fats. If biodiesel is to supply 5% of diesel usage, a government objective, first-time vegetable, likely from canola and soyabean, oil will also be required to provide...

Shin, E.; Hong, S-Won.; Kim, S.Hoon.; Yang, W-Ick., 2004: Expression of down stream molecules of RET (p-ERK, p-p38 MAPK, p-JNK and p-AKT) in papillary thyroid carcinomas. To evaluate the roles of 4 putative downstream molecules (ERK, p38 MAPK, JNK and AKT) of the RET signal pathway in the tumorigenesis of papillary carcinomas, the expression patterns of RET and phosphorylated forms, of ERK, p38 MAPK, JNK and AKT we...

Austin, M. P.; Meyers, J. A., 1996: Current approaches to modelling the environmental niche of eucalypts: implication for management of forest biodiversity. Robust predictive models of the distribution of forest biota are important tools for the management of forest biodiversity. Requirements for modelling the environmental niche of plant species include: environmentally stratified survey data of the...

Sismanis, A.; Butts, F.M., 1994: A practical device for detection and recording of objective tinnitus. Otolaryngology--Head and Neck Surgery 110(4): 459-462

Matsuda, M.; Tobari, Y.N., 2004: Genetic analyses of several Drosophila ananassae-complex species show a low-frequency major gene for parthenogenesis that maps to chromosome 2. Parthenogenetic strains of several species have been found in the genus Drosophila. The mode of diploidization in the eggs of females has been found to be post-meiotic nuclear fusion. The genetic basis for this parthenogenesis is not understood bu...

Ghaziuddin, M., 2000: Autism in Down's syndrome: A family history study. Several recent reports have described the occurrence of autism in subjects with Down's syndrome (DS). However, relatively little is known about the family history of these subjects, especially with reference to autism. In order to address thi...

Janetschek G.; Aufschnaiter M.; Bodner E., 1988: Percutaneous transhepatic gallstone removal by nephroscopic technique in a case of cholelithogenous liver abscess. In a patient with intrahepatic bile duct obstruction in the right lobe due to a retained stone, we succeeded in removing the calculus with a nephroscope through a percutaneous transhepatic puncture after a failed surgical attempt to extract the st...