EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of prostaglandin E and F production in the goldfish testis


, : Regulation of prostaglandin E and F production in the goldfish testis. Journal of Experimental Zoology 266(2): 108-115

The present studies have investigated the putative role of activators of different signal transduction pathways in the regulation of prostaglandin (PG) production by goldfish testis pieces in vitro. The protein kinase C activator (PKC) phorbol 12-myristate 13-acetate (PMA: 1.56-400 nM) and calcium ionophore A23187 (62.5-4000 nM) stimulated PGE and F production in a dose and time-dependent fashion. There was no synergism between A23187 and PMA in stimulating PG production. PGE levels were at least 4.5 times higher than PGF levels. The phorbol ester 4-alpha phorbol didecanoate (400 nM), which does not activate PKC, but had no effect on PGE production. The synthetic diacylglycerol 1-oleoyl-2-acetyl-sn-glycerol (100 mu-M), but not 1,2-dioctanoyl-sn-glycerol (100 mu-M), stimulated PGE production. Although the gonadotropin analog human chorionic gonadotropin (1 IU/ml), adenylate cyclase activator forskolin (2 mu-M) and testosterone (5 ng/ml) had no effect, the cyclic nucleotide phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (1 nM) and cyclic adenosine-3',5'-monophosphate analog dibutyryladenosine-3',5'-monophosphate (2 mM) inhibited PMA (400 nM) and A23187 (1 mu-M)-stimulated PGE production. PMA and A23187 (400 nM and 1 mu-M, respectively)-stimulated PGE production was inhibited by phospholipase A-2 inhibitors 4-bromophenacyl bromide, quinacrine and chloroquine (all 6.25-400 mu-M) and by diacylglycerol lipase inhibitor RHC 80267 (10-100 mu-M), suggesting that both lipases are involved in the release of precursor fatty acids for PG synthesis by goldfish testis tissue. These data, in conjunction with earlier studies showing that PGE stimulates testosterone production by goldfish testis pieces, lend support to the contention that locally produced PGs of the E series modulate testicular steroidogenesis in teleosts.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002477243

DOI: 10.1002/jez.1402660205

Download PDF Full Text: Regulation of prostaglandin E and F production in the goldfish testis


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Rosenblatt, H.M.; Hershfield, M.; Flood, T.J.; Gaspar, H.B., 1998: Thrombocytosis with enzyme replacement therapy in adenosine deaminase deficient severe combined immune deficiency. Journal of Allergy & Clinical Immunology 101(1 PART 2): S75, Jan

Snell, T. W., 1978: Fecundity developmental time and population growth rate. A computer simulation was used to investigate the relative effects of changes in net fecundity and developmental time on population growth rate (r). Increases in net fecundity had the largest effect on r when net fecundity is small. Conversely, de...

Nagahama, Y.; Fukuyama, H.; Yamauchi, H.; Katsumi, Y.; Dong, Y.; Konishi, J.; Kimura, J., 1997: Effect of nicardipine on cerebral blood flow in hypertensive patients with internal carotid artery occlusion: a PET study. The objective of this study was to elucidate the effect of intravenous administration of nicardipine on the cerebral blood flow of hypertensive patients with internal carotid artery occlusion (ICAO). Six patients with ICAO and 10 normal individual...

Steib A.; Freys G.; Ravanello J.; Curzola U.; Beller J.P.; Wolf P.; Ellero B.; Otteni J.C., 1989: Hemodynamic changes during liver transplantation with veno venous bypass effect of a preoperative hyperkinetic state. The routine use of a veno-venous bypass is not accepted by all surgical liver transplantation teams. The putative benefits (maintaining haemodynamic stability during the anhepatic phase, reducing blood losses, avoiding renal failure) are counterba...

Reimer G.; Drahovsky D., 1976: Chromosomal structures of pseudomonas testosteroni part 1 isolation and characterization of the chromosomal complexes. Zeitschrift fuer Naturforschung Teil C Biochemie Biophysik Biologie Virologie 31(1-2): 91-97

Edem, C., 1993: Midwifery in Nigeria. Midwifery Today and Childbirth Education: 23, 40-23, 40

Houssay, B.A.; Marenzi, A.D., 1931: Cortical adrenal extract and sensitiveness of adrenalectomised rats to intoxication. Swingle and Pfiffner's extract injected into adrenalectomised rats suppresses hypersensitiveness to morphine and histamine.

Leban, J. M., 1995: Estimating the sawmilling attributes of forest resources - application to Norway spruce (Picea abies). An account is given of the use of growth models to assess the quality of forest resources in terms of wood quality and output. The models use forest inventory data (stem increment, stem form, height growth, volume, branching), and data for Picea a...

Manning, G.; Millar Craig, M.W., 1993: 24 Hour non-invasive blood pressure monitoring Data from a randomly selected normal population. European Heart Journal 14(ABSTR SUPPL ): 142

Goyal, M.; Suresh, B.R.; Reinersman, G.; Gewolb, I.H.; Brion, L.P., 1995: Evolution and variability of pulmonary mechanics during postnatal transition in term infants. This study was designed to assess whether progressive changes related to the transition to extrauterine life explain in part the interassay variability of pulmonary mechanics in the immediate postnatal period. We performed sequential pulmonary fun...