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Regulation of prothoracic gland activity in diapausing larvae of the wax moth, Galleria mellonella L. (Lepidoptera)


, : Regulation of prothoracic gland activity in diapausing larvae of the wax moth, Galleria mellonella L. (Lepidoptera). Insect Biochemistry and Molecular Biology 23(1): 33-41

The development of Galleria mellonella is arrested by a lowered temperature of 18 degrees C at the stage of the last instar post-spinning larva. The haemolymph ecdysteroid titre of these larvae is maintained at a low level of ca 5 pg of 20-hydroxyecdysone per micro 1. Different types of surgical manipulations including nerve cord-severance, implantation of the brain, prothoracic glands (PGs) or fat body accompanied with ecdysteroid titre measurements show that the low ecdysteroid titre in diapausing larvae is due to nervous inhibition of their PGs. The inhibitory input probably comes from the brain and is transmitted down to the PGs via the suboesophageal ganglion and paired cervical nerves. Severance of the nerve tracts at any level caused rapid activation of PGs of diapausing insects. Nervous inhibition of the PGs ceases about 6-9 b after transfer of diapausing larvae from the lower temperature to the diapause-terminating conditions of 30 degrees C. As long as the PGs are under nervous inhibitory control they are refractory to humoral stimulation. The brains of diapausing larvae exhibit in vitro high prothoracicotropic activity which suggests that PTTH is accumulated in the brain during diapause. The role of juvenile hormone in inhibiting the release of PTTH from the brain to haemolymph, and its effect on the PGs during diapause is discussed.

Accession: 002477246

DOI: 10.1016/0965-1748(93)90079-8

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Related references

Mala, J.; Granger, N.A.; Sehnal, F., 1977: Control of prothoracic gland activity in larvae of galleria mellonella. Prothoracic glands of last instar wax moth larvae maintain spontaneous secretory activity in decapitated larvae and in isolated abdomens into which they have been transplanted, as judged by their ability to induce secretion of a new cuticle. Their...

Bohm, G-A.; Sobek, L.; Penzlin, H., 1988: Regulation of prothoracic gland activity in Galleria mellonella. Prace Naukowe Instytutu Chemii Organicznej I Fizycznej Politechniki Wroclawskiej Seria Monografie, 677-679 No. 33

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Vorontsova, I.L.; Ershov, N.I.; Glupov, V.V., 2006: An effect of the microsporidian Vairimorpha ephestiae (Microsporidia: Burenellidae) on activity and spectrum of nonspecific esterases in different tissues of the greater wax moth galleria mellonella (Lepidoptera: Pyralidae) larvae. The effect of Vairimorpha ephestiae on non-specific esterases in the haemolymph, fat body and midgut of larvae of Galleria mellonella was studied. Esterase patterns were analysed using polyacrylamide gel electrophoresis, whereas total esterase act...