EurekaMag.com logo
+ Translate

Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants


, : Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants. Plant molecular biology 24(4): 561-569

The abscisic acid-responsive gene rab17 is induced during maize embryo maturation and in vegetative tissues under water stress conditions. To investigate how ABA is involved in the induction of the rab17 gene, we present here a genetic approach to analyse the transcriptional regulation of the 1.3 kb rab17 promoter fragment in transgenic wild-type Arabidopsis and mutants which are deficient (aba) and insensitive (abi1, abi2 and abi3) to ABA. During seed development the rab17 promoter fragment confers similar temporal and spatial regulation on the reporter gene GUS, both in transgenic wild-type and ABA-deficient and ABA-insensitive mutants. The rab17 promoter was only active in embryo and endosperm during late seed development, although the ABA-deficient embryo mutant showed a reduction in the level of GUS activity. During germination rab17 promoter activity decreases, and GUS activity is not enhanced by water stress in transgenic wild-type and mutant plants. In contrast, transcription of the Arabidopsis endogenous rab gene is stimulated by water stress, both in wild-type and ABA-insensitive mutants. Our data suggest that different molecular mechanisms mediate seed-specific expression and ABA water stress induction of the rab17 gene and indicate strong conservation of the seed-specific regulatory mechanism for rab genes in monocot and dicot plants.

Accession: 002477308

PMID: 8155877

DOI: 10.1007/bf00023554

Download PDF Full Text: Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Vilardell, J.; Martínez-Zapater, J.M.; Goday, A.; Arenas, C.; Pagès, M., 1994: Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants. The abscisic acid-responsive gene rab17 is induced during maize embryo maturation and in vegetative tissues under water stress conditions. To investigate how ABA is involved in the induction of the rab17 gene, we present here a genetic approach to...

Goday, A.; Pla, M.; Niogret, F.; Jensen, A.; Figueras, M.; Pages, M., 1994: Regulation of the rab17 gene in ABA-deficient and ABA-insensitive viviparous mutants of maize. The effects of abscisic acid (ABA) and desiccation on the expression of the ABA responsive gene rab17 was studied in vp2 (viviparous) embryos, deficient in carotenoids and with low levels of ABA. Expression of rab17 was also studied in ABA-insensi...

Assmann, S.M.; Snyder, J.A.n; Lee, Y.R.J.lie, 2000: ABA-deficient and ABA-insensitive mutants of Arabidopsis have a wild-type stomatal response to humidity. In most plant species, a decrease in atmospheric humidity at the leaf surface triggers a decrease in stomatal conductance. While guard cells appear to respond to humidity-induced changes in transpiration rate, as opposed to relative humidity or va...

Assmann, S.M.; Snyder, J.A.; Lee YuhR.J., 2000: ABA-deficient (aba1) and ABA-insensitive (abi1-1, abi2-1) mutants of Arabidopsis have a wild-type stomatal response to humidity. In most plant species, a decrease in atmospheric humidity at the leaf surface triggers a decrease in stomatal conductance. While guard cells appear to respond to humidity-induced changes in transpiration rate, as opposed to relative humidity or va...

Vilardell, J.; Mundy, J.; Stilling, B.; Leroux, B.; Pla, M.; Freyssinet, G.; Pagès, M., 1991: Regulation of the maize rab17 gene promoter in transgenic heterologous systems. The maize rab17 gene is expressed in different plant parts in response to ABA and osmotic stress (J. Vilardell et al., Plant Mol Biol 14 (1990) 423-432). Here we demonstrate that 5' upstream sequences of the rab17 gene confer the appropriate...

Takahashi, T.; Naito, S.; Komeda, Y., 1992: The Arabidopsis HSP18.2 promoter/GUS gene fusion in transgenic Arabidopsis plants: a powerful tool for the isolation of regulatory mutants of the heat-shock response. Expression of the heat shock protein (HSP18.2) gene promoter from Arabidopsis fused to the bacterial GUS gene in transgenic Arabidopsis plants is described. High levels of GUS activity were induced in all organs of transformants except for seeds d...

Bianchi, M.W.; Damerval, C.; Vartanian, N., 2002: Identification of proteins regulated by cross-talk between drought and hormone pathways in Arabidopsis wild-type and auxin-insensitive mutants, axr1 and axr2. Ten proteins differentially regulated by progressive drought stress in A. thaliana Columbia wild type, axr1-3 and axr2-1 auxin-insensitive mutants, were identified from internal amino acid microsequencing. These proteins fell into two categories:...

Leymarie, J.; Damerval, C.; Marcotte, L.; Combes, V.; Vartanian, N., 1996: Two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axr1, axr2, reveal interactions between drought and hormonal responses. In order to detect gene products involved in Arabidopsis drought adaptive strategy, 2D-PAGE protein patterns of two auxin-insensitive mutants, axr1, axr2, differentially affected in specific drought responses, were compared to the wild-type Columb...

Leymarie, J.; Damerval, C.; Marcotte, L., 1996: Two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axrl, axr2, reveal interactions between drought and hormonal responses. Plant and Cell Physiology 37: 6-75

Kiss, J.Z.; Katembe, W.J.; Edelmann, R.E.; Kiss, J.Z., 2001: Gravitropism and development of wild-type and starch-deficient mutants of Arabidopsis during spaceflight. The "starch-statolith" hypothesis has been used by plant physiologists to explain the gravity perception mechanism in higher plants. In order to help resolve some of the controversy associated with ground-based research that has supporte...