EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants






Plant molecular biology 24(4): 561-569

Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants

The abscisic acid-responsive gene rab17 is induced during maize embryo maturation and in vegetative tissues under water stress conditions. To investigate how ABA is involved in the induction of the rab17 gene, we present here a genetic approach to analyse the transcriptional regulation of the 1.3 kb rab17 promoter fragment in transgenic wild-type Arabidopsis and mutants which are deficient (aba) and insensitive (abi1, abi2 and abi3) to ABA. During seed development the rab17 promoter fragment confers similar temporal and spatial regulation on the reporter gene GUS, both in transgenic wild-type and ABA-deficient and ABA-insensitive mutants. The rab17 promoter was only active in embryo and endosperm during late seed development, although the ABA-deficient embryo mutant showed a reduction in the level of GUS activity. During germination rab17 promoter activity decreases, and GUS activity is not enhanced by water stress in transgenic wild-type and mutant plants. In contrast, transcription of the Arabidopsis endogenous rab gene is stimulated by water stress, both in wild-type and ABA-insensitive mutants. Our data suggest that different molecular mechanisms mediate seed-specific expression and ABA water stress induction of the rab17 gene and indicate strong conservation of the seed-specific regulatory mechanism for rab genes in monocot and dicot plants.

Accession: 002477308

PMID: 8155877

DOI: 10.1007/bf00023554

Download PDF Full Text: Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants



Related references

Regulation of the rab17 gene in ABA-deficient and ABA-insensitive viviparous mutants of maize. Biochemical and cellular mechanisms of stress tolerance in plants Proceedings of the NATO advanced research workshop, Maratea, Italy, 20-24 June 1994: 335-343, 1994

ABA-deficient and ABA-insensitive mutants of Arabidopsis have a wild-type stomatal response to humidity. Plant Cell & Environment 23(4): 387-395, April, 2000

ABA-deficient (aba1) and ABA-insensitive (abi1-1, abi2-1) mutants of Arabidopsis have a wild-type stomatal response to humidity. Plant, Cell and Environment 23(4): 387-395, 2000

Regulation of the maize rab17 gene promoter in transgenic heterologous systems. Plant Molecular Biology 17(5): 985-993, 1991

The Arabidopsis HSP18.2 promoter/GUS gene fusion in transgenic Arabidopsis plants: a powerful tool for the isolation of regulatory mutants of the heat-shock response. Plant Journal 2(5): 751-761, 1992

Identification of proteins regulated by cross-talk between drought and hormone pathways in Arabidopsis wild-type and auxin-insensitive mutants, axr1 and axr2. Functional Plant Biology 29(1): 55-61, 2002

Two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axr1, axr2, reveal interactions between drought and hormonal responses. Plant & Cell Physiology 37(7): 966-975, 1996

Gravitropism and development of wild-type and starch-deficient mutants of Arabidopsis during spaceflight. Physiologia Plantarum 102(4): 493-502, 2001

Plastid sedimentation kinetics in roots of wild-type and starch-deficient mutants of Arabidopsis. Plant Physiology 120(1): 183-192, 1999

Features of the primary wall CESA complex in wild type and cellulose-deficient mutants of Arabidopsis thaliana. Journal of Experimental Botany 59(10): 2627-2637, 2008