EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Relatedness of the ericoid endophytes Scytalidium vaccinii and Hymenoscyphus ericae inferred from analysis of ribosomal DNA


Mycologia 85(2): 219-230
Relatedness of the ericoid endophytes Scytalidium vaccinii and Hymenoscyphus ericae inferred from analysis of ribosomal DNA
Phenotypic similarities between the root endophytes Scytalidium vaccinii, a hyphomycete, and Hymenoscyphus ericae, an ascomycete, led to speculation that S. vaccinii might be the anamorph of H. ericae. To test this hypothesis, we used the polymerase chain reaction to amplify portions of the ribosomal DNA from the ex-type cultures of each species and from cultures of several endophytes putatively identified as Scytalidium or H. ericae based on colonial and morphological similarities. An initial restriction fragment analysis of the amplified rDNA separated the H. ericae isolates from England from the ex-type culture of S. vaccinii and most isolates tentatively identified as H. ericae from North America. However, restriction mapping of the amplified fragment revealed that the apparent differences were an artifact resulting from the presence of one or two insertions in the small and large subunit ribosomal RNA genes of the North American isolates. Sequencing of homologous regions of the small subunit and the 5' internal transcribed spacer indicated that sequence divergence between pairs of isolates identified as Scytalidium and H. ericae was low, ranging from 1.2-3.5%, as compared to divergence of up to 24% with H. monotropae, another ericoid endophyte. Although there was variation among the isolates, molecular and morphological evidence suggests that S. vaccinii and H. ericae are anamorph and teleomorph states of a single taxon.


Accession: 002477477

DOI: 10.2307/3760460



Related references

Relatedness of the ericoid endophytes Scytalidium vaccinii and Hymenoscyphous ericae infereed from analysis of ribosomal DNA. Mycologia 85(2): 219-230, 1993

Using molecular data to infer anamorph-teleomorph connections The case of Scytalidium vaccinii and Hymenoscyphus ericae. Reynolds, D R , Taylor, J W The fungal holomorph: Mitotic, meiotic and pleomorphic speciation in fungal systematics : 141-146, 1993

Arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. New Phytologist 151(1): 265-270, 2001

Mechanism of arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. Plant Physiology 124(3): 1327-1334, 2000

Analysis of acid invertase and comparison with acid phosphatase in the ericoid mycorrhizal fungus Hymenoscyphus ericae (Read) Korf and Kernan. Mycorrhiza 2(2): 63-67, 1992

Utilization of sucrose by Hymenoscyphus ericae (an ericoid endomycorrhizal fungus) and ectomycorrhizal fungi. Mycological Research 99(10): 1233-1238, 1995

Purification and characterization of a beta-1,4-endoxylase from the ericoid mycorrhizal fungus Hymenoscyphus ericae. New Phytologist 135(2): 345-352, 1997

Purification and characterization of a beta -1,4-endoxylanase from the ericoid mycorrhizal fungus Hymenoscyphus ericae. New Phytologist 135(2): 345-352, 1997

Host range of a select isolate of the ericoid mycorrhizal fungus Hymenoscyphus ericae. HortScience 38(6): 1163-1166, 2003

Chitin degradation by Hymenoscyphus ericae and the influence of Hymenoscyphus ericae on the growth of ectomycorrhizal fungi. Read, D J [Editor], Lewis, D H [Editor], Fitter, A H [Editor], Alexander, I J [Editor] Mycorrhizas in ecosystems : 246-251, 1992