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Relationship between immunological properties and structural model of 11S rapeseed globulin


, : Relationship between immunological properties and structural model of 11S rapeseed globulin. Phytochemistry 0(11): 3511-3513

The immunodetection of rapeseed (Brassica napus) 11S globulin (cruciferin) constituents was investigated using two different rabbit immune sera raised against native cruciferin and against or and P free polypeptides constituting the cruciferin subunits. After two dimensional SDS-PAGE separation and transfer on immobilon membrane, the immunoblotting showed that only the alpha polypeptides reacted with anti-native cruciferin antibodies. The beta as well as alpha polypeptides displayed intensive immunoreactivity using anti-free polypeptides antibodies. These results are discussed with regard to the proposed model for the quaternary structure of the hexameric cruciferin molecule. The data were in agreement with the surface localization of the a polypeptides; P polypeptides would be buried within the cruciferin core.

Accession: 002477829

DOI: 10.1016/0031-9422(91)80056-7

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