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Role of chicken pancreatic trypsin, chymotrypsin and elastase in the excystation process of Eimeria tenella oocysts and sporocysts

Role of chicken pancreatic trypsin, chymotrypsin and elastase in the excystation process of Eimeria tenella oocysts and sporocysts

Journal of Protozoology Research 1(15): 22-26

The role of pancreatic proteolytic enzymes in the excystation process of Eimeria tenella oocysts and sporocysts was studied in vitro. Intact sporulated oocysts were preincubated in phosphate buffer, NaCl 0.9% (PBS), with 0.5% chicken bile extract in a 5% CO2 atmosphere for 30 minutes prior to exposure to either 0.25% (w/v) chicken trypsin, chymotrypsin, pancreatic elastase, or a 1% (w/v) crude extract of unsporulated and sporulated oocysts of E. tenella.

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