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Separation and identification of carotenoids and their oxidation products in the extracts of human plasma



Separation and identification of carotenoids and their oxidation products in the extracts of human plasma



Analytical Chemistry 64(18): 2111-2122



Eighteen carotenoids as well as vitamin A and two forms of vitamin E (gamma- and alpha-tocopherol) have been separated from extracts of human plasma by high-performance liquid chromatography (HPLC) on reversed-phase and silica-based nitrile-bonded columns. In the order of chromatographic elution on a C-18 reversed-phase column, the carotenoids were identified as (3R,3'R,6'R)-beta,epsilon-carotene-3,3'-diol ((3R,3'R,6'R)-lutein), (3R,3'R)-beta,beta-carotene-3,3'-diol ((3R,3'R)-zeaxanthin), 5,6-dihydroxy-5,6-dihydro-psi,psi-carotene, 3-hydroxy-2',3'-didehydro-beta,epsilon-carotene, beta, epsilon-caroten-3-ol, 3-hydroxy-beta-carotene, psi,psi-carotene, 7,8-dihydro-psi,psi-carotene, beta, psi-carotene, 7,8,7',8'-tetrahydro-psi,psi-carotene, beta,epsilon-carotene, beta,beta-carotene, 7,8,11,12,7',8'-hexahydro-psi,psi-carotene, and 7,8,11,12,7',8',- 11',12'-octahydro-psi,psi-carotene. The polar carotenoids, which eluted in the vicinity of lutein and were unresolved in the C-18 column, have been separated on a nitrile-bonded column employing isocratic HPLC conditions. In the order of elution, the carotenoids were epsilon,epsilon-carotene-3,3'-dione, 3'-hydroxy-epsilon,epsilon-caroten-3-one, 5,6-dihydroxy-5,6-dihydro-psi,psi-carotene, 3-hydroxy-beta,epsilon-caroten-3'-one, (all-E,3R,3'R,6'R)-lutein, (all-E,3R,3'R)-zeaxanthin, and (all-E,3R,3'S,6'R)-beta,epsilon-carotene-3,3'-diol (3'-epilutein) followed by several geometrical isomers of lutein and zeaxanthin.

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Accession: 002490274

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PMID: 1416048


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