Sequence of a tobacco (Nicotiana tabacum) gene coding for type a phytochrome

Adam, E.; Deak, M.; Kay, S.; Chua, N.H.; Nagy, F.

Plant Physiology 101(4): 1407-1408

1993


ISSN/ISBN: 0032-0889
PMID: 8310074
DOI: 10.1104/pp.101.4.1407
Accession: 002490490

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Abstract
Nucleotide and deduced amino acid sequences are reported for a genomic clone of the Nt-Phy-A1 gene (EMBL accession number X66784) from cv. NK326. The gene consists of a 960-bp 5'-untranslated region, a 4263-bp coding region and a 717 bp 3'-untranslated region. It is interrupted by 4 introns bounded by intron/exon splice junctions. The size of the deduced Nt-Phy-A1 sequence (4488 nucleotides) shows good correlation with the Nt-Phy-A1 transcript (4500 nucleotides) detected on Northern blots. The start codon is ATG and the (G + C) content in the coding region is 44.37%. The deduced 1125-amino acid protein shows high (76-92%) similarity to type-A phytochrome of other plant species (Arabidopsis thaliana, pea, potato).