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Spermiogenesis of the testes of juvenile hormone-treated silkworm larvae, Bombyx mori



Spermiogenesis of the testes of juvenile hormone-treated silkworm larvae, Bombyx mori



Comparative Biochemistry & Physiology A Comparative Physiology 106(3): 495-499



1. To examine changes in the percentages of testicular cells such as 1C cells (spermatids and spermatozoa), 2C cells (G1 somatic cells, spermatogonia and secondary spermatocytes), 2-4C cells (somatic and germinal cells in S phase) and 4C cells (G2 somatic cells and primary spermatocytes), we isolated nuclei from the testes of silkworm larvae and subjected them to flow cytometric analysis. 2. In control testes, 1C cells appeared at day 0 of the fifth instar, increased gradually by spinning and increased steeply at 2 days later still spinning. The percentage of 2C cells decreased gradually after ecdysis to the fifth instar. The percentage of 4C cells increased from day 2 to day 5 of the fifth instar and decreased after day 11 of this instar. Cells in S phase remained constant through the fifth instar. 3. An injection of juvenile hormone analog, methoprene, at day 0 of the fifth instar did not inhibit spermiogenesis, but resulted in increased 2C cells and decreased 4C cells dose-dependently. In contrast, the same treatment at day 2 of the fifth instar did not change the percentages of 1C, 2C and 4C cells of the testes at all, suggesting that the testes changed sensitivity to the hormone at larval development. 4. Repeated injections of methoprene to induce the appearance of dauer larvae resulted in a complete block of the development of 1C cells.

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Accession: 002497956

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DOI: 10.1016/0300-9629(93)90244-X


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