EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach



Targeted mapping and linkage analysis of morphological isozyme, and RAPD markers in peach



Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 87(7): 805-815



Nine different F2 families of peach [Prunus persica (L.) Batsch] were analyzed for linkage relationships between 14 morphological and two isozyme loci. Linkage was detected between weeping (We) and white flower (W), 33 cM; double flower (Dl) and pillar (Br), 10 cM; and flesh color (Y) and malate dehydrogenase (Mdh1), 26 cM. A leaf variant phenotypically distinct from the previously reported wavy-leaf (Wa) mutant in peach was found in progeny of 'Davie II'. The new willow-leaf character (designated Wa2) was closely linked (0.4 cM) to a new dwarf phenotype (designated Dw3). Two families derived from the pollen-fertile cultivar White Glory' segregated for pollen sterility, but segregation did not follow a 3:1 ratio. Evidence is presented suggesting that 'White Glory' possesses a pollen-sterility gene (designated Ps2) that is non-allelic to the previously reported pollen-sterility gene (Ps) in peach. Ps2 was linked to both weeping (We-Ps2 15.5 cM) and white flower Ps2- W, 25.3 cM). A genomic map of peach containing 83 RAPD, one isozyme, and four morphological markers was generated using an F2 family obtained by selfing an NC174RL x 'Pillar' Fl. A total of 83 RAPD markers were assigned to 15 linkage groups. Various RAPD markers were linked to morphological traits. Bulked segregant analysis was used to identify RAPD markers flanking the red-leaf (Gr) and Mdh1 loci in the NC174RL x 'Pillar' and 'Marsun' x 'White Glory' F2 families. respectively. Three markers flanking Mdh1 and ten markers flanking Gr were identified. The combination of RAPD markers and bulked segregant analysis provides an efficient method of identifying markers flanking traits of interest. Markers linked to traits that can only be scored late in development are potentially useful for marker-aided selection in trees. Alternatives for obtaining additional map order information for repulsion-phase markers in large F2 populations are proposed.

(PDF emailed within 0-6 h: $19.90)

Accession: 002509322

Download citation: RISBibTeXText

PMID: 24190466

DOI: 10.1007/BF00221132



Related references

Genetic linkage mapping in peach using morphological, RFLP and RAPD markers. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 90(3-4): 503-510, 1995

Genetic mapping in Xiphophorus hybrid fish: assignment of 43 AP-PCR/RAPD and isozyme markers to multipoint linkage groups. Genome Research 6(4): 280-289, 1996

Genetic mapping of new morphological, isozyme and RAPD markers in Vicia faba L. using trisomics. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 93(7): 1130-1138, 1996

Molecular-mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 93(7): 1017-1025, 1996

Molecular-mapping analysis in Brassica napus using isozyme, RAPD, RFLP markers on a double-haploid progeny. Theoretical & Applied Genetics 93(7): 1017-1025, 1996

A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea. Breeding Science 49(4): 257-265, 1999

Linkage among isozyme, RFLP and RAPD markers in Vicia faba. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 85(8): 937-945, 1993

An integrated genetic linkage map for eucalypts using RFLP, RAPD and isozyme markers. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 91(6-7): 869-875, 1995

Construction of a genetic linkage map in the wild plant Mimulus using RAPD and isozyme markers. Genome 39(1): 63-70, 1996

A linkage map construction for Chinese cabbage based on AFLP, SSR, RAPD and isozyme markers using DH population. Acta Horticulturae Sinica 32(2): 249-255, 2005

A general genetic linkage map for peach established by using RAPD and AFLP markers. Journal of Fruit Science 23(5): 766-769, 2006

A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers. Theoretical and Applied Genetics 102(8): 1190-1199, 2001

A genetic linkage map of Quercus robur L. (pedunculate oak) based on RAPD, SCAR, microsatellite, minisatellite, isozyme and 5S rDNA markers. Theoretical and Applied Genetics 97(7): 1090-1103, 1998