+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

The 5'-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression

The 5'-region of Arabidopsis thaliana cor15a has cis-acting elements that confer cold-, drought- and ABA-regulated gene expression

Plant Molecular Biology 24(5): 701-713

Previous nuclear run-on experiments indicated that the cor15a (cold-regulated) gene of Arabidopsis thaliana L. (Heyn) has a cold-inducible promoter (Hajela et al., Plant Physiol 93: 1246-1252, 1990). The data presented here indicate that the 5' region of cor15a between nucleotides -305 and +78 (relative to the start of transcription) contains a cis-acting element(s) that can impart cold-regulated gene expression. Histochemical staining experiments indicated that the cor15a promoter is inactive, or very weakly active, in most of the tissues and organs of plants grown at normal temperature and that it becomes activated throughout most of the plant in response to low temperature. Notable exceptions to this general pattern include constitutive activity of the promoter in anthers of control grown plants and apparent inactivity of the promoter in the roots and ovaries of cold-treated plants. Histochemical staining experiments also indicated that low temperature regulation of cor15a does not involve the synthesis of a regulatory molecule that can spread throughout the plant and induce cor gene expression at normal growth temperature. Finally, gene fusion experiments indicated that the 5' region of cor15a between nucleotides -305 and +78, in addition to imparting cold-regulated gene expression, can impart ABA- and drought-regulated gene expression.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002511255

Download citation: RISBibTeXText

PMID: 8193295

DOI: 10.1007/bf00029852

Related references

Consitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance. Proceedings of the National Academy of Sciences of the United States of America 93(23): 13404-13409, 1996

Cloning of the promoter fragment of a cold-inducible gene (cor15a) from Arabidopsis thaliana and its expression in transgenic potato. Journal of Agricultural Biotechnology 12(5): 500-504, 2004

Arabidopsis thaliana cor15b, an apparent homologue of cor15a, is strongly responsive to cold and ABA, but not drought. Plant molecular biology 23(5): 1073-1077, 1993

Cold acclimation and cold-regulated gene expression in ABA mutants of Arabidopsis thaliana. Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 17(6): 1233-1240, 1991

Effect of constitutive expression of the COR15a gene on the freezing tolerance of isolated protoplasts of Arabidopsis thaliana. Cryobiology 33(6): 656-657, 1996

Drought stress changes gus gene expression pattern regulated by TGG1 promoter in transgenic Arabidopsis thaliana L. Plant Physiology (Rockville) 108(2 Suppl. ): 39, 1995

Cold-regulated gene expression and freezing tolerance in an Arabidopsis thaliana mutant. Plant journal: for cell and molecular biology 17(3): 301-308, 1999

Expression and cis-acting elements of the At-beta-fruct1 gene from Arabidopsis thaliana encoding a cell wall invertase. Plant Physiology & Biochemistry 34(3): 431-442, 1996

A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana. Plant Science 163(1): 75-82, 2002

A role for circadian evening elements in cold-regulated gene expression in Arabidopsis. Plant Journal 60(2): 328-339, 2009