EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The carbachol-induced release of prothoracicotropic hormone from brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori






Journal of Insect Physiology 40(6): 469-473

The carbachol-induced release of prothoracicotropic hormone from brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori

The in vitro release of prothoracicotropic hormone (PTTH) from brain-corpus cardiacum-corpus allatum complex (brain-CC-CA complex) of Bombyx mori was stimulated significantly with carbachol (acetylcholine agonist) in the range of 0.01-10 mM. One mM muscarine also induced the release of PTTH within 5 min, while 1 mM nicotine exerted no effect. In addition, 5 mM atropine (muscarinic antagonist) inhibited the PTTH release induced with 0.1 mM carbachol. From these results, it became apparent that the muscarinic acetylcholine receptor was associated with the release of PTTH. To explore the signal transduction mediated by the muscarinic acetylcholine receptor in the PTTH release, the effects of phospholipase C (PLC)-inhibitor, calmodulin antagonist and protein kinase C (PKC)-inhibitor on the PTTH release induced with 0.1 mM carbachol were examined. 2-nitro-4-carboxyphenyl-N,N-diphenylcalbamate (NCDC: PLC-inhibitor), N-(6-aminohexyl)-5-chloro-1-1-naphthalenesulfonamide (W-7: calmodulin antagonist) and calphostin C (PKC-inhibitor) caused dose-dependent suppression of the carbachol-induced release of PTTH, and 10 mu-M NCDC, 500 mu-M W-7 and 10 mu-M calphostin C suppressed the release of PTTH completely. These results suggested that activation of phospholipase C, and subsequent protein phosphorylations by PKC and calcium/calmodulin dependent protein kinase (CaMK) were involved in the carbachol-induced signal transduction in the PTTH release.

Accession: 002513861

DOI: 10.1016/0022-1910(94)90119-8

Download PDF Full Text: The carbachol-induced release of prothoracicotropic hormone from brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori



Related references

The in vitro release of prothoracicotropic hormone (PTTH) from the brain-corpus cardiacum-corpus allatum complex of silkworm, Bombyx mori. Comparative Biochemistry and Physiology C Pharmacology Toxicology and Endocrinology 110(2): 143-148, 1995

Small GTP-binding proteins in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori: involvement in the secretion of prothoracicotropic hormone. Archives of Insect Biochemistry and Physiology 38(4): 177-184, 1998

Carbachol-induced protein phosphorylation in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori. Applied Entomology & Zoology 32(4): 573-581, 1997

The release of an insect neuropeptide hormone, bombyxin, is regulated by muscarinic transmission in the brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori (Lepidoptera: Bombycidae). Applied Entomology and Zoology 36(4): 431-438, 2001

Sexual difference in the activity of pars intercerebralis corpus cardiacum corpus allatum system of bombyx mori. Comptes Rendus Des Seances De La Societe De Biologie Et De Ses Filiales: 1174-1176, 1967

Identification of myotropic neuropeptides from the brain and corpus cardiacum-corpus allatum complex of the beetle, Zophobas atratus. Journal of Insect Science 10: 156-156, 2011

Brain-derived neurotrophic factor-like neuropeptide is secreted as a neurohormone from specific brain neurons into the corpus allatum in the silkworm Bombyx mori. Entomological Research 36(1): 16-19, 2006

Studies on the topography of corpus allatum-cardiacum complex and the volumetric changes of corpus allatum in relation to post embryonic development of ovary in Lucilia cuprina Wied (Diptera). Unknown, 1988

Function of the corpus allatum in the silkworm (Bombyx mori). Annotationes Zoologicae Japonenses, 24: 1-12, 1950

Allatostatins and allatotropin in the corpus cardiacum/corpus allatum complex of larval and adult lepidopterans studied by confocal laser scanning microscopy: correlation to juvenile hormone biosynthesis. Cell and Tissue Research 314(2): 281-295, 2003