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The carbachol-induced release of prothoracicotropic hormone from brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori



The carbachol-induced release of prothoracicotropic hormone from brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori



Journal of Insect Physiology 40(6): 469-473



The in vitro release of prothoracicotropic hormone (PTTH) from brain-corpus cardiacum-corpus allatum complex (brain-CC-CA complex) of Bombyx mori was stimulated significantly with carbachol (acetylcholine agonist) in the range of 0.01-10 mM. One mM muscarine also induced the release of PTTH within 5 min, while 1 mM nicotine exerted no effect. In addition, 5 mM atropine (muscarinic antagonist) inhibited the PTTH release induced with 0.1 mM carbachol. From these results, it became apparent that the muscarinic acetylcholine receptor was associated with the release of PTTH. To explore the signal transduction mediated by the muscarinic acetylcholine receptor in the PTTH release, the effects of phospholipase C (PLC)-inhibitor, calmodulin antagonist and protein kinase C (PKC)-inhibitor on the PTTH release induced with 0.1 mM carbachol were examined. 2-nitro-4-carboxyphenyl-N,N-diphenylcalbamate (NCDC: PLC-inhibitor), N-(6-aminohexyl)-5-chloro-1-1-naphthalenesulfonamide (W-7: calmodulin antagonist) and calphostin C (PKC-inhibitor) caused dose-dependent suppression of the carbachol-induced release of PTTH, and 10 mu-M NCDC, 500 mu-M W-7 and 10 mu-M calphostin C suppressed the release of PTTH completely. These results suggested that activation of phospholipase C, and subsequent protein phosphorylations by PKC and calcium/calmodulin dependent protein kinase (CaMK) were involved in the carbachol-induced signal transduction in the PTTH release.

Accession: 002513861

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DOI: 10.1016/0022-1910(94)90119-8

Download PDF Full Text: The carbachol-induced release of prothoracicotropic hormone from brain-corpus cardiacum-corpus allatum complex of the silkworm, Bombyx mori



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