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The development of a gene transfer system in the nematode Caenorhabditis elegans and definition of dye-coupling patterns in early Caenorhabditis elegans embryos



The development of a gene transfer system in the nematode Caenorhabditis elegans and definition of dye-coupling patterns in early Caenorhabditis elegans embryos



Dissertation Abstracts International B, Sciences and Engineering 51(3): 1121B



A technique for microinjection into Caenorhabditis elegans embryos has been developed and used for two purposes: introduction of DNA molecules into pre-germline cells with the goal of stable transformation, and introduction of the dye Lucifer Yellow into individual embryonic cells to define the pattern of dye coupling in early C. elegans embryos. Plasmid DNA injected into young embryos generate a frequency of transformation too low to be detected by Southern blot analysis.

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