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The presence of cucumber mosaic virus coat protein in chloroplasts is related to the expression of mosaic symptom on tobacco



The presence of cucumber mosaic virus coat protein in chloroplasts is related to the expression of mosaic symptom on tobacco



Acta Phytopathologica Sinica 22(3): 229-233



Intact chloroplasts were isolated from tobacco leaves infected by cucumber mosaic cucumovirus (CMV) or from transgenic tobacco leaves expressing CMV coat protein (CP). The CP was detected by Western blotting using antiserum against CMV CP. It is concluded that CP was found inside chloroplasts of tobacco infected with CMV; the concn of the CP inside the chloroplasts was positively related to the severity of the symptoms; and CP was not associated with chloroplasts from transgenic tobacco.

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