EurekaMag.com logo
+ Translate

The urinary excretion of thiobarbituric acid reactive substances and malondialdehyde by normal adult males after consuming a diet containing salmon


, : The urinary excretion of thiobarbituric acid reactive substances and malondialdehyde by normal adult males after consuming a diet containing salmon. Lipids 28(8): 757-761

In this study we investigated the output of thiobarbituric acid reactive substances (TBARS) and malondialdehyde (MDA), as thiobarbituric acid (TBA)-MDA adduct, in the urine from subjects eating a diet in which the only source of n-3 long-chain, polyunsaturated fatty acids was fresh salmon. Nine healthy men, ages 30-65, were confined in the United States Department of Agriculture Western Human Nutrition Research Center, San Francisco, CA, for 100 d; food intake and exercise levels were controlled. All subjects were placed on a stabilization diet (StD) for 20 d, then six were fed the salmon diet for 40 d. The others remained on the StD. The groups switched diets for the primarily eicosapentaenoic acid and docosahexaenoic acid in a 50:60 ratio, while the StD contained 7.5% from n-6 FAs and lt 0.3% n-3 FAs (with presumably no significant amounts of C-20 or C-22 n-3 FAs). Twenty-four hour urinary output was collected, and 2% 3-d pool samples prepared for analysis of urinary TBARS and the TBA-MDA adduct. The total urinary output of each individual varied considerably, and on a daily basis the concentration of autoxidation products in an individual's urine varied also. However, the mean daily output (in mu-moles TBA-MDA equivalents/day) at the end of the salmon diet feeding period was significantly greater (7.05 +- 1.33 TBARS, P lt 0.05; and 7.07 +- 1.73 TBA-MDA adduct, P lt 0.01) compared to when the subjects were eating the StD (5.65 +- 1.09 TBARS and 4.65 +- 0.76 TBA-MDA adduct). When the TBARS and TBA-MDA adduct values were normalized relative to creatinine output (in nmoles TBA-MDA equivalents/mu-mole creatinine), the data achieved even greater statistical significance. The mean output of the group eating the salmon diet was 0.478 +- 0.076 for TBARS (P lt 0.01) and 0.476 +- 0.082 for the TBA-MDA adduct (P lt 0.001) vs. 0.345 +- 0.059 for TBARS and 0.283 +- 0.041 for the TBA-MDA adduct when the subjects were consuming the StD. Thus, the consumption of cooked fish may increase one's exposure to AMA and other autoxidation products, compounds that may be carcinogenic or mutagenic.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002530013

PMID: 8377591

DOI: 10.1007/bf02536000

Download PDF Full Text: The urinary excretion of thiobarbituric acid reactive substances and malondialdehyde by normal adult males after consuming a diet containing salmon


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Merkoçi, A., 2011: Nanoparticles-based strategies for DNA, protein and cell sensors. The need for novel biosensing systems has increased enormously in the last few years. In this context nanoparticles with special optical and electrochemical properties are bringing significant advantages in fields such as clinical analysis, enviro...

Cordisco, CA.; Trotta, P.; Romanelli, M., 2005: Spawning plasticity of baby clam Chamelea gallina Linnaeus, 1758 Plasticita riproduttiva della vongola comune Chamelea gallina Linneo, 1758. This study examines the gonadal maturation cycle Chamelea gallina during all year. About 500 individuals were observed, in vivo, from sampling areas in Central-Southern Adriatic Sea (Molise and Northern Apulia) and in Central Tyrrhenian Sea (Latiu...

Kullmann, W., 1986: Glass ionomer cements--physical-technical properties in relation to their preparation. Deutsche Zahnarztliche Zeitschrift 41(8): 751-754

Vechiato, M.H.; Maringoni, A.C.; Martins, E.M.F., 2006: Development of primers and method for identification and detection of Diaporthe phaseolorum var. meridionalis in soybean seeds. In spite of the great variability of the Diaporthe/Phomopsis complex, the routine seed health testing, used for detection of the Diaporthe, is not reliable, taking consideration into that for its identification morphological characteristics of the...

Anonymous, 1987: Legal issues affecting nursing. State Nursing Legislation Quarterly 5(1): 28-30

Tenhagen, B.A.; Birkelbach, E.; Heuwieser, W., 2000: Serum progesterone levels in post-partum dairy cows after repeated application of the prostaglandin F2 alpha analogue D (+) cloprostenol sodium. Reproductive management programmes based on strategic use of prostaglandin F2 alpha (PGF2 alpha) to induce and synchronize oestrus in post-partum dairy cows are widespread. Repeated shortening of the oestrous cycle during early lactation in high-y...

Foster, G.A.; Healy, T.R.; De-Lange, W.P., 1996: Presaging beach renourishment from a nearshore dredge dump mound, Mt. Maunganui Beach, New Zealand. A study was undertaken to ascertain whether material to be dredged from the shipping channel through a large ebb tidal delta at the entrance to Tauranga Harbor, New Zealand, could be dumped in shallow water (5-8 m below Mean Sea Level) off the dow...

Wang, X.; Huang, J.; Mukherjee, A.; Cao, C.; Lutkenhaus, J., 1997: Analysis of the interaction of FtsZ and itself, GTP, and FtsA. The interaction of FtsZ with itself, GTP, and FtsA was examined by analyzing the sensitivity of FtsZ to proteolysis and by using the yeast two-hybrid system. The N-terminal conserved domain consisting of 320 amino acids bound GTP, and a central re...

Sajwani, R.A.; Shoukat, S.; Raza, R.; Shiekh, M.Muhyeuddin.; Rashid, Q.; Siddique, M.Saulat.; Panju, S.; Raza, H.; Chaudhry, S.; Kadir, M., 2009: Knowledge and practice of healthy lifestyle and dietary habits in medical and non-medical students of Karachi, Pakistan. To objectively compare the differences in knowledge and practices regarding healthy lifestyle among medical and non-medical students of Karachi along with assessment of any perceived barriers. This cross-sectional study included 350 students betwe...

Staiger, D., 2002: Circadian rhythms in Arabidopsis: time for nuclear proteins. To be prepared for the periodic alterations of light and darkness in their environment, plants utilise an endogenous clock to anticipate these changes and to time physiological processes appropriately. Large-scale screens for mutants with perturbe...