EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum


, : The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum. Current genetics5(5): 438-444

RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A molecular study of 49 RAPDs obtained showed that, with only one exception, all RAPD markers studied represent repetitive DNA. This situation precludes the direct use of these markers to either initiate chromosome walking to a gene of interest or for the assignment of linkage groups to electrophoretically-separated chromosomes. A simple reamplification test has been applied which permitted the quick discrimination between single-copy and repetitive DNA species, without the need for Southern analysis. Additionally, evidence is shown for the presence of single-stranded DNA species in the amplification products, and that these may be present in addition to their double-stranded counterparts. The reamplification test can also identify these DNA species, avoiding misinterpretation of polymorphic bands.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 002530070

PMID: 8082190

DOI: 10.1007/bf00351783

Download PDF Full Text: The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Wang, C.; Cao, J.C., 2005: Current oscillation and chaotic dynamics in superlattices driven by crossed electric and magnetic fields. We have theoretically studied current oscillation and chaotic dynamics in doped GaAsAlAs superlattices driven by crossed electric and magnetic fields. When the superlattice system is driven by a dc voltage, a stationary or dynamic electric-field d...

Palaniyar, N.; Ridsdale, R.A.; Possmayer, F.; Harauz, G., 1998: Surfactant protein A (SP-A) forms a novel supraquaternary structure in the form of fibers. We have found by transmission electron microscopy that bovine surfactant protein A (SP-A) formed extended fibers in the presence of calcium. On phosphatidylcholine or especially dipalmitoylphosphatidylcholine monolayers, SP-A at roughly 0.005 mg/m...

Whyte, L., 1965: The growing importance of the use of ultrasonics in medicine. Elektro Medizin; Biomedizin und Technik 10(4): 213-216

Montarsi, F.; Martini, S.; Dal Pont, M.; Delai, N.; Ferro Milone, N.; Mazzucato, M.; Soppelsa, F.; Cazzola, L.; Cazzin, S.; Ravagnan, S.; Ciocchetta, S.; Russo, F.; Capelli, G., 2014: Distribution and habitat characterization of the recently introduced invasive mosquito Aedes koreicus [Hulecoeteomyia koreica], a new potential vector and pest in north-eastern Italy. The container breeding species belonging to the genus Aedes (Meigen) are frequently recorded out of their place of origin. Invasive Aedes species are proven or potential vectors of important Arboviruses and their establishment in new areas pose a...

Jacobson, G.A.; Diba, K.; Yaron-Jakoubovitch, A.; Oz, Y.; Koch, C.; Segev, I.; Yarom, Y., 2005: Subthreshold voltage noise of rat neocortical pyramidal neurones. Neurones are noisy elements. Noise arises from both intrinsic and extrinsic sources, and manifests itself as fluctuations in the membrane potential. These fluctuations limit the accuracy of a neurone's output but have also been suggested to p...

Ganguly, Nandita, D., 2007: Trend of tropospheric ozone in Indian forests from 1980 to 2000. The seasonal and yearly trends of tropospheric ozone for a few Indian forests from 1980 to 2000 have been studied using ozone data obtained from the tropospheric ozone residual method. The season in which peak value of ozone concentration is obser...

Bryant, C.; Beall, M.; McPhaul, L.; Fortson, W.; Ross, M., 2006: Do placental sections accurately reflect umbilical cord nucleated red blood cell and white blood cell differential counts?. Elevated levels of umbilical cord nucleated red blood cells (nRBCs) have been used to assess in utero hypoxia. Although the umbilical nRBC value has been the 'gold standard', umbilical blood may not be obtained at delivery. We determined...

Mendelson, N.H.; Sarlls, J.E.; Thwaites, J.J., 2001: Motions caused by the growth of Bacillus subtilis macrofibres in fluid medium result in new forms of movement of the multicellular structures over solid surfaces. Bacillus subtilis macrofibres, highly ordered multicellular structures, undergo twisting and writhing motions when they grow in fluid medium as a result of forces generated by the elongation of individual cells. Macrofibres are denser than the flu...

Xiang, Q.; Tan, L.; Zhao, Y.; Wang, J.; Luo, A., 2008: Ketamine: the best partner for isoflurane in neonatal anesthesia?. Isoflurane is one of the most commonly used inhalation anesthetic in neonatal anaesthesia. It has been suggested that isoflurane can induce caspase activation and apoptosis when applied in a clinically relevant concentration in the developing brai...

Gressner, A.M.; Wool, I.G., 1974: The stimulation of the phosphorylation of ribosomal protein S6 by cycloheximide and puromycin. Biochemical and Biophysical Research Communications 60(4): 1482-1490