+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The use of a digoxigenin-labelled synthetic DNA oligonucleotide for the rapid and sensitive identification of Meloidogyne incognita

Fundamental & Applied Nematology 16(6): 495-499

The use of a digoxigenin-labelled synthetic DNA oligonucleotide for the rapid and sensitive identification of Meloidogyne incognita

Accurate identification of Meloidogyne species is essential for effective control and management strategies. Variable morphology and the existence of intraspecific races make most current diagnostic procedures difficult and unreliable. This work describes the use of a 30 base non-radioactive digoxigenin-labelled synthetic oligonucleotide to identify M. incognita in Slot Blot assays. The sensitivity of this approach is such that it is capable of detecting as little as 2 ng of M. incognita genomic DNA. Preliminary attempts to characterize the 50 base M. incognita specific probe on which this oligonucleotide was based indicated that it could represent approximately 1% of the haploid genome of M. incognita and is repeated 17 000 times.

(PDF 0-2 workdays service: $29.90)

Accession: 002530098

Related references

PCR assays for rapid and sensitive identification of three major root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria. Acta Phytopathologica Sinica 34(3): 204-210, 2004

Rapid chemiluminescent detection of the mRNA for uncoupling protein in brown adipose tissue by northern hybridization with a 32-mer oligonucleotide end-labelled with digoxigenin. International Journal of Obesity and Related Metabolic Disorders 18(6): 449-452, 1994

A species-specific oligonucleotide DNA probe for the identification of Meloidogyne incognita. Parasitology 103 Pt 2: 315-319, 1991

HLA class II typing in a microtitre plate formate using digoxigenin-labelled amplified DNA and biotin-labelled oligonucleotide probes. European Journal Of Immunogenetics. 20(5): 419-427, 1993

Rapid confirmation of Listeria monocytogenes isolated from foods by a colony blot assay using a digoxigenin-labeled synthetic oligonucleotide probe. Applied and Environmental Microbiology 57(6): 1609-1614, 1991

Identification and metabolism of indoles in Meloidogyne incognita and in cotton resistant and susceptible to Meloidogyne incognita. Dissertation Abstracts International 34B(2): 484, 1973

A rapid and sensitive dot-blot hybridization assay for the detection of citrus exocortis viroid in Citrus medica with digoxigenin-labelled RNA probes. Journal of Virological Methods 57(2): 203-207, 1996

Determination of HLA-B alleles by digoxigenin-labelled oligonucleotide probes and comparison with serological result. Human Immunology 39(2): 134, 1994

Demonstration of DNA fingerprints in cattle and chickens by using the digoxigenin-labelled oligonucleotide probe (GGAT)4. Wiener Tierarztliche Monatsschrift 80(10): 302-305, 1993

Reaction of Hibiscus rosa-sinensis cultivars to two species of root knot nematodes Meloidogyne incognita, Meloidogyne incognita, Meloidogyne javanica. HortScience 18(1): 85-86, 1983