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The use of a digoxigenin-labelled synthetic DNA oligonucleotide for the rapid and sensitive identification of Meloidogyne incognita


, : The use of a digoxigenin-labelled synthetic DNA oligonucleotide for the rapid and sensitive identification of Meloidogyne incognita. Fundamental & Applied Nematology 16(6): 495-499

Accurate identification of Meloidogyne species is essential for effective control and management strategies. Variable morphology and the existence of intraspecific races make most current diagnostic procedures difficult and unreliable. This work describes the use of a 30 base non-radioactive digoxigenin-labelled synthetic oligonucleotide to identify M. incognita in Slot Blot assays. The sensitivity of this approach is such that it is capable of detecting as little as 2 ng of M. incognita genomic DNA. Preliminary attempts to characterize the 50 base M. incognita specific probe on which this oligonucleotide was based indicated that it could represent approximately 1% of the haploid genome of M. incognita and is repeated 17 000 times.

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