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The use of a monoclonal antibody against bluetongue virus non-structural protein NS1 in a competitive assay for the detection of anti-tubule antibodies

, : The use of a monoclonal antibody against bluetongue virus non-structural protein NS1 in a competitive assay for the detection of anti-tubule antibodies. Bluetongue, African horse sickness, and related orbiviruses: Proceedings of the Second International Symposium: 589-595

A MAb directed against BLU virus non-structural protein NS1 was used in a competitive (C) ELISA to detect antibodies to tubules (composed of protein NS1) in serum samples. Anti-tubule antibodies were detected at approximately 10 days after infection in BLU virus-infected sheep, but no such antibodies were detected in sheep injected with inactivated BLU virus vaccines. Tubules are also produced during the replication of other related orbiviruses.

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