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The use of double stranded RNAs to diagnose citrus tristeza virus strains


, : The use of double stranded RNAs to diagnose citrus tristeza virus strains. Turkiye II Biyolojik mucadele kongresi bildirileri Proceedings of the second turkish national congress of biological control, 26-29 September, 1990: 119-125

dsRNAs were extracted from citrus tristeza closterovirus (CTV)-infected citrus tissue and examined using agarose gel electrophoresis. Each CTV isolate had a full-length replicative form (RF) and several shorter subgenomic dsRNAs. The number and size of each subgenomic dsRNA were unique for each CTV isolate.

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Related references

Lee, R.F., 1984: Use of double stranded RNAs to diagnose citrus tristeza virus strains. The dsRNAs were purified from CTV-infected bark tissue by the use of CF-11 cellulose followed by polyacrylamide gel electrophoresis. The sizes of dsRNAs purified from 5 CTV isolates which differed widely in their biological activity were compared....

Dodds J.A.; Jarupat T.; Roistacher C.N.; Lee J.G., 1987: Detection of strain specific double stranded rnas in citrus species infected with citrus tristeza virus a review. Major and minor dsRNAs of citrus tristeza virus (CTV) were detected and compared with confidence when the dsRNA purified from 2,0 g of infected bark tissue was analysed by polyacrylamide gel electrophoresis. Virus specific dsRNAs were detected in...

Moreno, P.G.erri, J.M.noz, N., 1990: Identification of Spanish strains of citrus tristeza virus by analysis of double-stranded RNA. Double-stranded RNA (dsRNA) patterns of 24 Spanish isolates of citrus tristeza virus (CTV) representing a wide range of biological diversity were examined. Seven distinct patterns, differing by the number and/or position of the dsRNA bands, were o...

Moreno, P.; Guerri, J.; Ballester Olmos, J.F.; Albiach, R.; Martinez, M.E., 1993: Separation and interference of strains from a citrus tristeza virus isolate evidenced by biological activity and double-stranded RNA (dsRNA) analysis. Separation of strains of citrus tristeza closterovirus (CTV), differentiated by their dsRNA profiles, was obtained by graft-inoculating citron plants from a Mexican lime that had been recently aphid- or graft-inoculated with a mild CTV isolate (T-...

Korkmaz, S., 2002: Investigation of biological properties, double-stranded RNA patterns and antigen concentration in citrus species infected with citrus tristeza virus. Biological properties and dsRNA patterns of one Cyprus and three Turkish isolates of citrus tristeza virus (CTV) were investigated. In addition, CTV antigen concentration and effect of tissue sampling time from naturally infected, field-grown &apo...

Gowda, S.S.tyanarayana, T.A.llon, M.; Albiach-Marti, M.; Mawassi, M.R.bindran, S.G.rnsey, S.; Dawson, W., 2001: Characterization of the cis-acting elements subgenomic mRNAs of Citrus tristeza virus: production of positive- and negative-stranded 3-terminal and positive-stranded 5-terminal RNAs. Virology 2001 286(1): 134-151

Dodds J.A.; Jordan R.L.; Roistacher C.N.; Jarupat T., 1987: Diversity of citrus tristeza virus isolates indicated by double stranded rna analysis. One major dsRNA of molecular weight (MW) 13.3 .times. 106 and two others (MW 1.9 .times. 106 and 0.8 .times. 106) were routinely detected by polyacrylamide gel electrophoresis in extracts from sweet orange (Citrus sinensis) or citron (Citrus medic...

Gowda, S.; Satyanarayana, T.; Ayllón, M.A.; Albiach-Martí, M.R.; Mawassi, M.; Rabindran, S.; Garnsey, S.M.; Dawson, W.O., 2001: Characterization of the cis-acting elements controlling subgenomic mRNAs of citrus tristeza virus: production of positive- and negative-stranded 3'-terminal and positive-stranded 5'-terminal RNAs. Citrus tristeza virus (CTV), a member of the Closteroviridae, has an [similar or equal]20-kb positive-sense RNA genome with two 5' ORFs translated from the genomic RNA and 10 3' genes expressed via nine or ten 3'-terminal subgenomic...

Dodds J.A.; Jarupat T.; Lee J.G.; Roistacher C.N., 1987: Effects of strain host time of harvest and virus concentration on double stranded rna analysis of citrus tristeza virus. Major and minor double-stranded ribonucleic acids (dsRNAs) of citrus tristeza virus (CTV) could be detected and compared with confidence when all the dsRNAs purified from 1 to 2 g of infected citrus bark tissue were analyzed by polyacrylamide gel...

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