+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The use of immobilized lectins in the separation of Staphylococcus aureus, Escherichia coli, Listeria and Salmonella spp. from pure cultures and foods

, : The use of immobilized lectins in the separation of Staphylococcus aureus, Escherichia coli, Listeria and Salmonella spp. from pure cultures and foods. Journal of Applied Bacteriology 73(1): 41-52

Lectins from Helix pomatia, Canavalia ensiformis, Agaricus bisporus and Triticum vulgaris agglutinated cultures of Staphylococcus aureus, Escherichia coli, Listeria and Salmonella spp. This agglutination was specific as it was inhibited (except with A. bisporus lectin) by the competing sugar substrates. The ability of three of these lectins, immobilized on a variety of supports, to separate these micro-organisms from pure cultures was investigated. Immobilization of the lectins on magnetic microspheres was the most effective method. Immobilized T. vulgaris lectin bound 87-100% of cells from cultures of L. monocytogenes, 80-100% of Staph. aureus, 33-45% of Salmonella spp. and 42-77% of E. coli. The A. bisporus lectin bound 31-63% of cells in cultures of L. monocytogenes, 83% of Staph. aureus but only 3-5% of the salmonella cells. Similarly H. pomatia lectin bound > 92% of Staph. aureus and 64% of L. monocytogenes cells but was poor at binding the Gram-negative organisms. This preference for binding Gram-positive organisms was confirmed when mixed cultures were studied. The T. vulgaris lectin was effective in removing L. monocytogenes (43%) and Staph. aureus (26%) from diluted milk and Salmonella (31-54%) from raw egg. Agaricus bisporus lectin removed L. monocytogenes from undiluted milk (10-47%) or ground beef (32-50%).

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002530369

DOI: 10.1111/j.1365-2672.1992.tb04967.x

Download PDF Full Text: The use of immobilized lectins in the separation of Staphylococcus aureus, Escherichia coli, Listeria and Salmonella spp. from pure cultures and foods

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Muren, A., 1967: Prolonged continuous anaesthesia in the dog. Acta Anaesthesiologica Scandinavica 11(3): 231-236

Hemre G I.; Lambertsen G.; Lie O., 1991: The effect of dietary carbohydrate on the stress response in cod gadus morhua. 150 cod (mean weight = 440 g) were divided into two groups and fed diets with or without carbohydrate for 60 days. No differences were found in total growth during the feeding period. Both fish groups were then stressed by handling and transported...

Francis J. E., 1991: Palaeoclimatic significance of Cretaceous-early Tertiary fossil forests of the Antarctic Peninsula. International Symposium on Antarctic Earth Sciences 5: 623-627

Kiselev A.Y.; Dolenko G.N., 1989: Epicenters for petroleum genesis in the Dnieper-Donets Basin. Geologicheskiy Zhurnal (Kiev, 1968) 1989(1): 9-16

Coreno, G.; Tangari, F., 1977: Personal experience with desamino-oxytocin in the induction of labor. Rivista Italiana di Ginecologia 58(4): 305-317

KING, M.V., 1958: On the thermal melting and recrystallization of ribonuclease crystals and the heterogeneity of ribonuclease. Various crystalline forms of bovine pancreatic ribonuclease, heated in their mother liquors, melt to form gels at temperatures characteristic of the crystal forms used. On incubation at 25[degree]C, crystals grow again in the samples, usually of t...

Harley R.M., 1974: New collections of labiatae from brazil notes on new world labiatae part 3. Kew Bulletin 29(1): 125-140

DE SAINT-MARTIN, R., 1957: Preoperative hypotonia maxima in surgery of the eyeball. Annales D'oculistique 190(3): 165-186

Sheets, J.L.; Wilcox, C.; Wilwerding, T., 2007: Cement selection for cement-retained crown technique with dental implants. The purpose of this study was to assess and compare the retentive nature of common dental cements that have been adapted for use in the implant abutment cement-retained crown (CRC) technique with those specifically formulated for this purpose. Ten...

Csernansky, J., G.; Newcomer, J., W.; Jackson, K.; Lombrozo, L.; Faull, K., F.; Zipursky, R.; Pfefferbaum, A.; Faustman, W., O., 1994: Effects of raclopride treatment of plasma and CSF HVA: Relationships with clinical improvement in male schizophrenics. Thirty-two acutely psychotic, male schizophrenic patients received raclopride, at 2, 6, or 12 mg/day, or haloperidol, 15 mg/day for 4 weeks after randomized, double-blind assignment. Twenty-six patients, including 19 who had been assigned one of t...