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The use of recombinant DNA methods to produce schistosome antigens in order to assess their immunodiagnostic potential: Schistosoma mansoni tropomyosin an example


, : The use of recombinant DNA methods to produce schistosome antigens in order to assess their immunodiagnostic potential: Schistosoma mansoni tropomyosin an example. Immunodiagnostic approaches in schistosomiasis Proceedings of ICGEB/TDR Symposium, Shanghai, 1990: 125-132

It was shown that the recombinant S. mansoni tropomyosin shows species specificity in a limited series of samples tested in both ELISA and Western blot analysis. It did not cross react with sera from S. haematobium or S. japonicum or other parasitic diseases. This finding suggests that S. mansoni tropomyosin warrants further study into its potential for immunodiagnosis.

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Xu, H.; Rekosh, D.M.; Andrews, W.; Higashi, G.I.; Nicholson, L.; Loverde, P.T., 1991: Schistosoma mansoni tropomyosin: production and purification of the recombinant protein and studies on its immunodiagnostic potential. A cDNA that encodes Schistosoma mansoni tropomyosin, except for 10 amino acids at the amino terminus, has been cloned into a pOTSNCO plasmid vector. Induced expression resulted in a constant level of recombinant protein production. The recombinant...

Nicholson L.J.; X.H.; Thakur A.N.; Rekosh D.M.; Loverde P.T., 1991: Schistosoma mansoni tropomyosin a species specific immunodiagnostic reagent. American Journal of Tropical Medicine & Hygiene 45(3 SUPPL): 159

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