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The use of the modified dry blot procedure for RFLP analysis






Maize Genetics Cooperation Newsletter ( 67): 116-117

The use of the modified dry blot procedure for RFLP analysis

An adaptation of the modified dry blot technique for RFLP analysis of maize DNA is described. Starting from DNA digestion, the amount of time to detect single-copy sequences with 32P-dCTP nick translocated probes is reduced to a minimum of 4 days. Gene Screen Plus membranes can be stripped and rehybridized 8 or more times, maintaining signal intensity without increasing background.

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Accession: 002530638



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