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The use of the modified dry blot procedure for RFLP analysis


, : The use of the modified dry blot procedure for RFLP analysis. Maize Genetics Cooperation Newsletter ( 67): 116-117

An adaptation of the modified dry blot technique for RFLP analysis of maize DNA is described. Starting from DNA digestion, the amount of time to detect single-copy sequences with 32P-dCTP nick translocated probes is reduced to a minimum of 4 days. Gene Screen Plus membranes can be stripped and rehybridized 8 or more times, maintaining signal intensity without increasing background.

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Other references

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Arnold, A.C.; Haman, K.; Garland, E.M.; Raj, V.; Dupont, W.D.; Biaggioni, I.; Robertson, D.; Raj, S.R., 2014: Cognitive dysfunction in postural tachycardia syndrome. Mental clouding is an almost universal complaint among patients with postural tachycardia syndrome (POTS) but remains poorly understood. Thus, we have determined whether POTS patients exhibit deficits during neuropsychological testing relative to...

Fong, P.; Lirman, D., 1995: Hurricanes cause population expansion of the branching coral Acropora palmata Wound healing and growth patterns of asexual recruits. Three mechanisms aiding recovery and expansion of a population of Acropora palmata on a patch reef that was directly in the path of Hurricane Andrew were documented: rapid wound healing, high rates of asexual recruitment, and rapid growth rate of...

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Sun, X.; Zhang, K.; Ji, X.; Wang, Y.; Jeffrey, Z.; Tong, Y.; Gao, H.; Zhang, J.; Wang, Z., 2012: Screening of pancreatic lipase and alpha-glucosidase inhibitors from Chinese dietary herbs. The present study was conducted to develop new inhibitors of pancreatic lipase and alpha-glucosidase from Chinese dietary herbs. Sixty-three dietary herbs from 39 taxonomic families were selected and extracted with aqueous ethanol or water. The ex...