+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

The use of the Emu promoter with antibiotic and herbicide resistance genes for the selection of transgenic wheat callus and rice plants

Australian Journal of Plant Physiology 21(1): 95-112
The use of the Emu promoter with antibiotic and herbicide resistance genes for the selection of transgenic wheat callus and rice plants
Coding sequences of selectable marker genes were introduced into a plasmid containing the Emu promoter sequence which drives high levels of expression in cereal cells. The selectable markers were those coding for the enzymes neomycin phosphotransferase (NPT*) for kanamycin resistance, hygromycin phosphotransferase (HPT) for hygromycin resistance, phosphinothricin N-acetyltransferase (PAT) for resistance to the herbicidal compound phosphinothricin, and a mutant acetolactate synthase (ALS) for resistance to sulfonylurea herbicides. Plasmid DNAs were introduced into wheat and rice protoplasts by electroporation. Wheat protoplast-derived callus lines producing NPT and PAT were selected with kanamycin and phosphinothricin respectively. Wheat callus transformed with the gene coding for the mutant ALS was selected with sulfometuron methyl following the bombardment of suspension cultures with DNA-coated microparticles. With rice, transformed plants were regenerated from protoplast-derived cultures selected with kanamycin and hygromycin. Integration of the introduced DNA in transformed rice plants was confirmed by gel blot hybridisation, and significant levels of the NPT enzyme were measured by ELISA in leaves of the primary transformants as well as in leaves, seeds and roots of progeny plants. The results confirm that efficient selection of transformed cereal cultures and plants can be achieved by using selectable genes linked to the Emu promoter.

(PDF same-day service: $19.90)

Accession: 002530641

DOI: 10.1071/PP9940095

Related references

Comparison of visual screening, herbicide and antibiotic selection of transgenic oat plants. In Vitro Cellular & Developmental Biology Animal 36(3 Part 2): 47 A, March, 2000

Herbicide resistant transgenic rice (Oryza sativa L.) plants obtained by microprojectile bombardment of regenerable embryogenic callus. Environment and Ecology 16(4): 950-953, 1998

The impact of selection parameters on the phenotype and genotype of transgenic rice callus and plants. Transgenic Research 4(1): 44-51, 1995

Selection of transgenic rice plants using a herbicide tolerant form of the acetolactate synthase gene. Methods in Molecular Biology 847: 59-66, 2012

Herbicide resistance of transgenic rice plants expressing human CYP1A1. Biotechnology Advances 25(1): 75-84, 2006

Breeding of transgenic rice lines with GNA and Bar genes resistance to both brown planthopper and herbicide. Acta Phytophylacica Sinica 34(5): 555-556, 2007

Advances in the selection of transgenic plants using non-antibiotic marker genes. Physiologia Plantarum 111(3): 269-272, 2001

Herbicide resistance in transgenic plants with mammalian P450 monooxygenase genes. Pest Management Science 61(3): 286-291, 2005

Self-fertile transgenic wheat plants expressing bromoxynil herbicide resistance. Acta Botanica Sinica 38(12): 942-948, 1996

Self-fertile transgenic wheat plants expressing herbicide bromoxynil resistance. Acta Botanica Sinica 38(12): 942-948, 1996