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The use of the competitive ELISA to detect neutralizing antibodies resulting from superinfection with a heterotypic bluetongue virus serotype

, : The use of the competitive ELISA to detect neutralizing antibodies resulting from superinfection with a heterotypic bluetongue virus serotype. Bluetongue, African horse sickness, and related orbiviruses: Proceedings of the Second International Symposium: 617-625

The blocking or competitive (c) ELISA using a group-specific MAb is widely used to detect previous exposure to BLU viruses, but it cannot identify subsequent infection with another serotype of BLU virus. A cELISA incorporating MAb specific for the 5 USA serotypes of BLU virus is described, which is able to detect this humoral response.

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