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The use of the fluorochrome DAPI in the diagnosis of lethal disease of coconut palm (Cocos nucifera) in Tanzania


, : The use of the fluorochrome DAPI in the diagnosis of lethal disease of coconut palm (Cocos nucifera) in Tanzania. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz 99(6): 614-616

The fluorochrome DAPI (4'-6-diamidino-2-phenylindole) binds with DNA to form fluorescent complexes under UV light, enabling the presence of DNA in cells infected by MLO and viruses to be demonstrated. This technique has been recommended for diagnosis of lethal disease of coconut palm in Tanzania, an MLO-associated disease. However, its use was complicated by the occurrence of fluorescence in unstained sections of diseased tissue.

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