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The viability of thawed cattle blastocysts produced by in vitro fertilization using two methods adapted for practical conditions


, : The viability of thawed cattle blastocysts produced by in vitro fertilization using two methods adapted for practical conditions. Journal of Reproduction and Development 38(5): 15-19

In the 1st method, embryos were equilibrated with 1.36 M glycerol and 0.25 M trehalose for 10-15 min in modified phosphate-buffered saline (mPBS) supplemented with 20% cow serum. Embryos were loaded into 0.25-ml plastic straws (2 embryos per straw) in a small volume of freezing medium separated by 2 air bubbles from the medium (0.25 M trehalose with 20% cow serum in mPBS). The straws were placed in a chamber kept at -7 degrees C, and then seeded and held for 10 min at this temp.

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