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The viability of thawed cattle blastocysts produced by in vitro fertilization using two methods adapted for practical conditions

, : The viability of thawed cattle blastocysts produced by in vitro fertilization using two methods adapted for practical conditions. Journal of Reproduction and Development 38(5): 15-19

In the 1st method, embryos were equilibrated with 1.36 M glycerol and 0.25 M trehalose for 10-15 min in modified phosphate-buffered saline (mPBS) supplemented with 20% cow serum. Embryos were loaded into 0.25-ml plastic straws (2 embryos per straw) in a small volume of freezing medium separated by 2 air bubbles from the medium (0.25 M trehalose with 20% cow serum in mPBS). The straws were placed in a chamber kept at -7 degrees C, and then seeded and held for 10 min at this temp.

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Related references

Goto K.; Takuma Y.; Matsuura S.; Nakanishi Y.; Yanagida K.; Katahira K.; Toguchi M.; Nonaka K., 1992: A study on the viability of bovine blastocysts derived from in vitro fertilization after freezing and thawing by two cryopreservation methods adapted to practical use in the field. Two cryopreservation methods adapted to field use were investigated with bovine blastocysts produced by in vitro technique. In Method I, embryos were equilibrated with 1.36M glycerol and 0.25M trehalose for 10-15 min in modified phosphate buffered...

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