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Use of an indirect haemagglutination test for the detection of Clostridium perfringens type A enterotoxin



Use of an indirect haemagglutination test for the detection of Clostridium perfringens type A enterotoxin



Memorias do Instituto Oswaldo Cruz 86(1): 25-29



An indirect haemagglutination (IH) test is described for the detection of Clostridium perfrringens type A enterotoxin, produced by strains isolated from human cases of food poisoning and from contaminated food. Though no strict relaitonship could be observed between titers in the IH test add the time it took mice to die from the intravenous inoculation of mice (IIM test), results of the supernatants examined by both methods demonstrated that the IH test was the more sensitive than the IIM one. No unspecific reaction was obtained in the IH with a negative control and the inhibitions of the IH and IIM tests by specific antiserum against C. perfringens enterotoxin showed that the IH test is very specific. The IH assay is recommended for its sensitivity and easy performance by less-equipped labororaties, by these and other data.

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Accession: 002539815

Download citation: RISBibTeXText

PMID: 1842397

DOI: 10.1590/S0074-02761991000100005



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