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Zinc deficiency in rat does not alter the major histone patterns in liver nuclei

Nutrition Research 13(5): 541-548

Zinc deficiency in rat does not alter the major histone patterns in liver nuclei

Zinc deficiency exerts many effects on DNA structure, metabolism and function. Others have observed that in the single-celled Euglena gracilis, zinc deficiency causes the distinctive appearance of a class of 3-5-kilodalton (kDa) polypeptides. The major histones, excepting H4, are released from chromatin of zinc deficient Euglena only when these 3-5-kDa polypeptides are removed. The purpose of this study was to determine whether zinc deficiency in rat is associated with a similar class of nuclear polypeptides and the accompanying increased affinity of the major histones to chromatin. Proteins were extracted from purified liver nuclei by two methods, 0.25 M hydrochloric acid (HCl) or 5% perchloric acid (PCA). There was no evidence of a class of 3-5-kDa polypeptides from nuclei of zinc deficient rats. There was also no qualitative change in the major histones. Although there were significant decrease in the amount of HCl and PCA-extracted proteins (P lt 0.0001) from zinc-deficient liver nuclei, the differences are eliminated when DNA content is taken into account.

Accession: 002549121

DOI: 10.1016/S0271-5317(05)80681-5

Download PDF Full Text: Zinc deficiency in rat does not alter the major histone patterns in liver nuclei

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