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Zinc deficiency in rat does not alter the major histone patterns in liver nuclei


, : Zinc deficiency in rat does not alter the major histone patterns in liver nuclei. Nutrition Research 13(5): 541-548

Zinc deficiency exerts many effects on DNA structure, metabolism and function. Others have observed that in the single-celled Euglena gracilis, zinc deficiency causes the distinctive appearance of a class of 3-5-kilodalton (kDa) polypeptides. The major histones, excepting H4, are released from chromatin of zinc deficient Euglena only when these 3-5-kDa polypeptides are removed. The purpose of this study was to determine whether zinc deficiency in rat is associated with a similar class of nuclear polypeptides and the accompanying increased affinity of the major histones to chromatin. Proteins were extracted from purified liver nuclei by two methods, 0.25 M hydrochloric acid (HCl) or 5% perchloric acid (PCA). There was no evidence of a class of 3-5-kDa polypeptides from nuclei of zinc deficient rats. There was also no qualitative change in the major histones. Although there were significant decrease in the amount of HCl and PCA-extracted proteins (P lt 0.0001) from zinc-deficient liver nuclei, the differences are eliminated when DNA content is taken into account.

Accession: 002549121

DOI: 10.1016/S0271-5317(05)80681-5

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Related references

Castro, C.E.; Alvares, O.F.; Sevall, J.S., 1986: Zinc deficiency decreases histone HI0 in rat liver. For 4 weeks male Sprague-Dawley rats initially weighing 50 g were fed on a synthetic zinc-deficient (Zn 0.9 mg/kg) diet or that diet with ZnCO3 40 mg/kg, pair-fed or fed to appetite. The H1 family of proteins was isolated from the liver and separa...

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