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Beta -Glucan synthesis in the cotton fiber. IV. In vitro assembly of the cellulose I allomorph


, : Beta -Glucan synthesis in the cotton fiber. IV. In vitro assembly of the cellulose I allomorph. Plant Physiology 107(1): 111-123

In vitro assembly of cellulose from plasma membrane extracts of cotton fibre was enriched by a combination of 3-(N-morpholino)propanesulfonic acid extraction buffer and 2 independent digitonin solubilization steps consisting of 0.05% digitonin (SE1) followed by 1% digitonin (SE2). Glucan synthase activity assays revealed that, although the SE2 fraction possessed higher activity, only 8.6% of the in vitro product survived acetic/nitric acid treatment.

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