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'Race no more': an alternative approach to cloning the 5' end of transcripts


, : 'Race no more': an alternative approach to cloning the 5' end of transcripts. Nucleic Acids Research 22(16): 3427-3428

A new polymerase chain reaction approach is described which allows successful isolation and cloning of the sequences at the 5' termini of transcripts as an alternative to the less straightforward RACE (rapid amplification of cDNA ends) protocols and commercial kits currently available. The protocol was developed to isolate the 5' region of the relatively rare transcript encoding the murine alpha M290 protein.

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Accession: 002549551

PMID: 7521528

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