EurekaMag.com logo
+ Translate

1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice


, : 1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice. FEBS Letters 342(3): 291-296

Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant of milk because of abdominal symptoms.

Accession: 002549645

PMID: 8150087

DOI: 10.1016/0014-5793(94)80519-9

Download PDF Full Text: 1 kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Troelsen, J.T.; Mehlum, A.; Olsen, J.; Spodsberg, N.; Hansen, G.H.; Prydz, H.; Noren, O.; Sjostrom, H., 1994: Kb of the lactase-phlorizin hydrolase promoter directs post-weaning decline and small intestinal-specific expression in transgenic mice. Adult-type hypolactasia is a genetic condition making approximately one half of the human population intolerant to milk because of abdominal symptoms. The cause is a post-weaning down-regulation of the intestinal-specific enzyme lactase-phlorizin...

Krasinski, S.D.; Upchurch, B.H.; Irons, S.J.; June, R.M.; Mishra, K.; Grand, R.J.; Verhave, M., 1997: Rat lactase-phlorizin hydrolase/human growth hormone transgene is expressed on small intestinal villi in transgenic mice. Background and Aims: Lactase-phlorizin hydrolase (LPH) is an absorptive enterocyte-specific gene that is expressed in a well-characterized pattern along the cryptvillus (vertical), proximal-distal (horizontal), and developmental (temporal) gradien...

Krasinski, S.D.; Upchurch, B.H.; Irons, S.J.; Mishra, K.; Grand, R.J.; Verhave, M., 1994: Appropriate cell- and crypt/villus-specific expression of the rat lactase-phlorizin hydrolase gene in transgenic mice. Gastroenterology 106(4 SUPPL ): A244

Wacker, H.; Keller, P.; Falchetto, R.; Legler, G.; Semenza, G., 1992: Location of the two catalytic sites in intestinal lactase-phlorizin hydrolase. Comparison with sucrase-isomaltase and with other glycosidases, the membrane anchor of lactase-phlorizin hydrolase. Lactase-phlorizin hydrolase was isolated by immunoadsorption chromatography from rabbit brush-border membrane vesicles. Inactivation of the enzyme with [3H]conduritol-B-epoxide, a covalent active site-directed inhibitor, labeled glutamates at posi...

Krasinski, S.D.; Gielen, M.; Dowling, L.N.; Bosse, T.; Van Wering, H.M.; Akiyama, T.E.; Gonzalez, F.J.; Montgomery, R.K.; Grand, R.J., 2003: HEPATOCYTE NUCLEAR FACTOR-1alpha DIFFERENTIALLY REGULATES LACTASE-PHLORIZIN HYDROLASE GENE EXPRESSION DURING WEANING IN MICE. Hepatocyte nuclear factor-1alpha (HNF-1alpha) is known to bind and activate the promoters of target intestinal genes in vitro, including lactase-phlorizin hydrolase (LPH). However, little is known about the importance of HNF-1alpha for the regulat...

Tsuboi, K.K.; Kwong, L.K.; Sunshine, P.; Castillo, R.O., 1992: Mechanism of maturational decline of rat intestinal lactase-phlorizin hydrolase. The maturational decline in lactase-phlorizin hydrolase (LPH) activity was studied in groups of young rats ranging from suckling to early post-weaned states. Associated maturational increases in sucrase-isomaltase (SI) and maltase-glucoamylase (MG...

Aihara, H.; Hiwatashi, N.; Kinouchi, Y.; Shimosegawa, T.; Toyota, T., 1999: T3b promoter directs specific expression on intestinal epithelial cells in transgenic mice. Gastroenterology 116(4 PART 2): A853, April

Aihara, H.; Hiwatashi, N.; Kumagai, S.; Obata, Y.; Shimosegawa, T.; Toyota, T.; Miyazaki, J.I.hi, 1999: The T3b gene promoter directs intestinal epithelial cell-specific expression in transgenic mice. Although a few promoters that direct intestinal epithelial cell-specific expression in transgenic animals have been reported, they are not necessarily appropriate for transgenic studies in terms of activity and tissue specificity. Here, we examine...

Aihara, H.; Hiwatashi, N.; Kumagai, S.; Obata, Y.; Shimosegawa, T.; Toyota, T.; Miyazaki, J., 1999: The T3(b) gene promoter directs intestinal epithelial cell-specific expression in transgenic mice. Although a few promoters that direct intestinal epithelial cell-specific expression in transgenic animals have been reported, they are not necessarily appropriate for transgenic studies in terms of activity and tissue specificity. Here, we examine...

Troelsen, J.T.; Mitchelmore, C.; Olsen, J., 2003: An enhancer activates the pig lactase phlorizin hydrolase promoter in intestinal cells. Lactase phlorizin hydrolase is a small intestinal-specific brush border protein commonly used as a specific marker of differentiated enterocytes. A number of transcription factors involved in the enterocyte-specific expression of lactase phlorizin...